Evidence map›Paper›PMID 39855299›Full record

ArticleJournal of advanced research2025

Integrated multi-omics reveals the Bacillus amyloliquefaciens BA40 against Clostridium perfringens infection in weaned piglets.

Zipeng Jiang, Weifa Su, Mingzhi Yang, Jie Fu, Tao Gong, Wentao Li, Chaoyue Wen, Xinxia Wang, Fengqin Wang, Mingliang Jin and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Temperature regulation mechanisms of diapause inFrontiers in microbiology · 2026
    Article
  5. Animals : an open access journal from MDPI · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Zipeng JiangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Weifa SuKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Mingzhi YangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Jie FuKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Tao GongKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Wentao LiKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Chaoyue WenKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Xinxia WangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Fengqin WangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Mingliang JinKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China.
Yizhen WangKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China. Electronic address: yzwang321@zju.edu.cn.
Zeqing LuKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China. Electronic address: zqlu2012@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionClostridium perfringens (C. perfringens) can cause necrotic enteritis and higher mortality rates in piglets, by impairing the intestinal barrier function. Bacillus amyloliquefaciens 40 (BA40) has showed potential ability to reduce C. perfringens infections, but the mechanisms responsible for its effectiveness remain unclear.

objectivesThis study aims to evaluate the impact of BA40 on inflammation induced by C. perfringens and to explain the mechanisms underlying its therapeutic effects. We aim to show how BA40 can bolster piglet health by strengthening the intestinal barrier and regulating immune responses.

methodsWe used piglets and cellular models, alongside microbiomics, metabolomic, and transcriptomic analyses, to investigate BA40's impact on C. perfringens-induced inflammation. A model of C. perfringens infection was constructed using piglets and cells to investigate the effect of BA40 on its phenotype. Microbiomics, metabolomics, and transcriptomics analyses were subsequently used to investigate the mechanisms of protection and immune response to BA40 on the intestinal barrier of piglets.

resultsOur study revealed significant improvements in piglet health following BA40 administration. Notably, BA40 strengthened the intestinal mucosal barrier and mitigated the inflammatory response triggered by C. perfringens BA40 decreased harmful bacteria and increased beneficial bacteria. Metabolite profiles improved, showing a reduction in harmful substances. Transscriptomics analysis indicated BA40's role in TNF/NF-κB signaling pathway, hinting at its ability to regulate immune responses and reduce intestinal inflammation. Cellular assays further confirmed BA40's capacity to diminish inflammatory cytokine release and encourage the differentiation of anti-inflammatory macrophages.

conclusionDatasets from the present study demonstrate that BA40 modulates gut microbes and metabolites, inhibits inflammation-related signaling pathways, and maintains gut barrier function. Our findings not only deepen our understanding of the therapeutic capacity of BA40 but also provide a theoretical foundation for the development of probiotics and alternative therapies aimed at improving piglet gut health.

Indexed as

Bacillus amyloliquefaciensClostridium InfectionsClostridium perfringensProbioticsSwine DiseasesAnimalsGastrointestinal MicrobiomeIntestinal MucosaMetabolomicsMultiomicsSwineTranscriptomeWeaningBacillus amyloliquefaciensCellClostridium perfringensGut immunityMulti-omicsPiglets

Identifiers

PMID39855299
PMCPMC12627879

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.