Evidence map›Paper›PMID 39856554›Full record

ArticleBMC genomics2025

BDH1 identified by transcriptome has a negative effect on lipid metabolism in mammary epithelial cells of dairy goats.

Mengke Ni, Saige Zang, Yihan Wang, Xiaochen Qin, Min Tian, Tiantian Xiong, Chong Chen, Yue Zhang, Jun Luo, Cong Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. BDH1 acetylation at K116 modulates milk fat production in dairy goats.Journal of animal science and biotechnology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mengke NiCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Saige ZangCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Yihan WangCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Xiaochen QinCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Min TianCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Tiantian XiongCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Chong ChenCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Yue ZhangCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.
Jun LuoCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China. luojun@nwafu.edu.cn.
Cong LiCollege of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China. congl@nwafu.edu.cn.

Funding

Key Research and Development Program of Shaanxi Province 2018ZDCXL-NY-01-05Shaanxi Livestock and Poultry Breeding Double-chain Fusion Key Project 2022GD-TSLD-46The Key Project of Innovation 2030 of Science and Technology 2022ZD0401401The National Natural Science Foundation of China 31702098
6 · The paper itself

Abstract

backgroundThe 3-hydroxybutyrate dehydrogenase 1 (BDH1) mainly participates in the regulation of milk fat synthesis and ketone body synthesis in mammary epithelial cells. In our previous study, BDH1 was identified as a key candidate gene regulating lipid metabolism in mammary glands of dairy goats by RNA-seq. This study aimed to investigate the effect of BDH1 on lipid metabolism in mammary epithelial cells of dairy goats (GMECs).

resultsThe results suggest that BDH1 plays a significant role in reducing triacylglycerol content and lipid droplet accumulation in GMECs (p < 0.05). Overexpression of BDH1 significantly decreased the expression of lipid metabolism-related genes (SREBF1 and GPAM) and reduced the levels of C14:0 and C17:1, while increasing FABP3 expression and C10:0 concentration (p < 0.05). Interference with BDH1 significantly increased the expression of SREBF1 and GPAM and the concentration of C14:0, C15:1, and C20:1, but significantly decreased FABP3 and C18:0 (p < 0.05). Treatment of GMECs with β-hydroxybutyric acid (R-BHBA) significantly decreased the expression of FASN, ACACA, LPL, SREBF1, FABP3, ACSL1, GPAM, DGAT1, and triacylglycerol content, while significantly increasing the expression of BDH1 (p < 0.05). Interference with BDH1 rescued the reduction of cellular TAG content and the expression of FASN, LPL, SREBF1, ACSL1, and GPAM in BHBA-treated GMECs.

conclusionIn conclusion, BDH1 negatively regulates lipid metabolism in mammary glands of dairy goats. Furthermore, it may mitigate the inhibitory effect of R-BHBA on lipid metabolism in GMECs.

Indexed as

Epithelial CellsGoatsHydroxybutyrate DehydrogenaseLipid MetabolismMammary Glands, AnimalTranscriptomeAnimalsFemaleGene Expression ProfilingTriglyceridesHydroxybutyrate DehydrogenaseTriglyceridesBDH1Dairy goatsExpression regulationLipid metabolismMammary epithelial cells

Identifiers

PMID39856554
PMCPMC11761236

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.