Evidence map›Paper›PMID 39862430›Full record

ArticleSTAR protocols2025

Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry.

Johannes Krell, Komal Kumar Javarappa, Angie Wenedy, Andrew L Frelinger, Laurent Renia, Clarissa Prazeres da Costa, Martin Schlegel, Percy Knolle, Gerhard Schneider, Oliver Hayden

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Johannes KrellDepartment of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, Germany. Electronic address: johannes.krell@mri.tum.de.
Komal Kumar JavarappaTUM CREATE Ltd, CREATE Way 10-02, CREATE Tower, Singapore.
Angie WenedyDepartment of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, Germany; TUM CREATE Ltd, CREATE Way 10-02, CREATE Tower, Singapore.
Andrew L FrelingerCenter for Platelet Research Studies, Dana-Farber/Boston Children's Cancer and Blood Disorders Center, Harvard Medical School, Boston, MA, USA.
Laurent ReniaA∗STAR Infectious Diseases Labs, Agency for Science, Technology and Research (A∗STAR), Singapore, Singapore; Lee Kong Chuan School of Medicine, Nanyang Technological University, Singapore, Singapore.
Clarissa Prazeres da CostaGerman Center for Infection Research (DZIF), partner site Munich, Germany; Center for Global Health, School of Medicine and Health, TUM, München, Germany; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, School of Medicine and Health, TUM, München, Germany.
Martin SchlegelDepartment of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, Germany.
Percy KnolleInstitute of Molecular Immunology, University Hospital München rechts der Isar, School of Medicine and Health, 81675 München, Germany.
Gerhard SchneiderDepartment of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, Germany.
Oliver HaydenHeinz-Nixdorf-Chair of Biomedical Electronics, TranslaTUM, School of Computation, Information and Technology, TUM, Germany; Munich Institute of Biomedical Engineering, TUM, Germany. Electronic address: oliver.hayden@tum.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets.

Indexed as

Cell AggregationFlow CytometryImage CytometryBlood PlateletsHumansLeukocytesClinical ProtocolFlow CytometryImmunologyMicroscopy

Identifiers

PMID39862430
PMCPMC11969411

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.