ArticleBMC microbiology2025
Metatranscriptomic time series insight into antibiotic resistance genes and mobile genetic elements in wastewater systems under antibiotic selective pressure.
Article in BMC microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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Who cites it
3 citing papers in PubMed.
- Bovine Mastitis Therapy at a Crossroads: Pharmacokinetic Barriers, Biofilms, Antimicrobial Resistance, and Emerging Solutions.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Monitoring Cross-Border Dissemination of Antimicrobial Resistance (AMR) via Air Travel to Saudi Arabia: Formulation of Monitoring Strategies to Mitigate AMR Concerns.Environmental science & technology · 2026Article
- Unraveling the activity of phage-carrying antibiotic resistance genes in constructed wetlands.Frontiers in cellular and infection microbiology · 2026Article
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Authors and funding
6 authors.
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Abstract
backgroundWastewater systems are usually considered antibiotic resistance hubs connecting human society and the natural environment. Antibiotic usage can increase the abundance of both ARGs (antibiotic resistance genes) and MGEs (mobile gene elements). Understanding the transcriptomic profiles of ARGs and MGEs remains a major research goal.
methodsBatch experiments were conducted by adding ampicillin to fresh activated sludge to final concentrations of 100 and 20 mg/L. The ampicillin concentration was detected via UPLC‒MS/MS. Metatranscriptomic data from the samples in the reactors were detected in a time series (0 to 10 h). The transcription profiles of ARGs and MGEs were summarized by searching metatranscriptomic datasets against related databases.
resultsDuring incubation, the dosed ampicillin could be removed completely within 8.5 h and 4 h, following first-order biodegradation kinetics models (R
conclusionThe P14171 gene was the only key ARG that responded to ampicillin degradation in the tested activated sludge systems, indicating its ability and potential health risk. External ampicillin could accelerate HGT in activated sludge systems by increasing the transcription of MGEs.
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Registered trials
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