Evidence map›Paper›PMID 39868181›Full record

ArticlebioRxiv : the preprint server for biology2025

Development of FAP-targeted theranostics discovered by next-generation sequencing-augmented mining of a novel immunized VNAR library.

Gihan S Gunaratne, Joseph P Gallant, Kendahl L Ott, Payson L Broome, Sasha Celada, Jayden L West, Jason C Mixdorf, Eduardo Aluicio-Sarduy, Jonathan W Engle, Eszter Boros and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Gihan S GunaratneDepartment of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0002-1094-5820
Joseph P GallantDepartment of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0003-4943-1744
Kendahl L OttDepartment of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0009-0004-1369-6550
Payson L BroomeDepartment of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.
Sasha CeladaDepartment of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.
Jayden L WestDepartment of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0003-1852-5457
Jason C MixdorfDepartment of Medical Physics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0003-3045-5607
Eduardo Aluicio-SarduyDepartment of Medical Physics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0002-7889-9162
Jonathan W EngleDepartment of Medical Physics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0002-3399-7228
Eszter BorosDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.ORCID 0000-0002-4186-6586
Labros MeimetisDepartment of Radiology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0002-5304-2066
Joshua M LangDepartment of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.ORCID 0000-0002-0943-8872
Shuang G ZhaoUniversity of Wisconsin Carbone Cancer Center, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.
Reinier HernandezDepartment of Medical Physics, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0002-0729-2179
David KosoffDepartment of Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin.ORCID 0000-0001-8559-6357
Aaron M LeBeauDepartment of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI 53705, USA.ORCID 0000-0003-3802-7789

Funding

Targeting CD133 for imaging and therapy in prostate cancerR01CA237272 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI LEBEAU, AARON MATTHEW · 2019 to 2023
$1.8M
Novel Radioimmunotherapy Strategies for Prostate CancerR01CA233562 · NCI · UNIVERSITY OF WISCONSIN-MADISON · PI LEBEAU, AARON MATTHEW · 2019 to 2023
$1.7M
CSRD VA I01 CX002479NCI NIH HHS R01 CA233562NCI NIH HHS R01 CA237272
6 · The paper itself

Abstract

Cancer-associated fibroblasts (CAFs) in the stroma of solid tumors promote an immunosuppressive tumor microenvironment (TME) that drives resistance to therapies. The expression of the protease fibroblast activation protein (FAP) on the surface of CAFs has made FAP a target for development of therapies to dampen immunosuppression. Relatively few biologics have been developed for FAP and none have been developed that exploit the unique engagement properties of Variable New Antigen Receptors (VNARs) from shark antibodies. As the smallest binding domain in nature, VNARs cleverage unique geometries and recognize epitopes conventional antibodies cannot. By directly immunizing a nurse shark with FAP, we created a large anti-FAP VNAR phage display library. This library allowed us to identify a suite of anti-FAP VNARs through traditional biopanning and also by an

Identifiers

PMID39868181
PMCPMC11761682

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.