Evidence map›Paper›PMID 39868845›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2025

High-Viability Circulating Tumor Cells Sorting From Whole Blood at Single Cell Level Using Laser-Induced Forward Transfer-Assisted Microfiltration.

Qingmei Xu, Yuntong Wang, Songtao Dou, Yang Xu, Zhenhe Xu, Han Xu, Yi Zhang, Yanming Xia, Ying Xue, Hang Li and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Qingmei XuSchool of Integrated Circuits, Peking University, Beijing, 100871, China.ORCID https://orcid.org/0000-0002-6685-5082
Yuntong WangChangchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China.ORCID https://orcid.org/0000-0002-4911-9446
Songtao DouSchool of Integrated Circuits, Peking University, Beijing, 100871, China.
Yang XuCancer Translational Medicine Research Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116027, China.
Zhenhe XuState Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai, 200237, China.ORCID https://orcid.org/0000-0003-2639-6181
Han XuSchool of Integrated Circuits, Peking University, Beijing, 100871, China.ORCID https://orcid.org/0000-0002-2742-5354
Yi ZhangSchool of Integrated Circuits, Peking University, Beijing, 100871, China.
Yanming XiaGuangzhou National Laboratory, Guangzhou, 510320, China.
Ying XueHooke Laboratory, Changchun, 130033, China.
Hang LiHooke Laboratory, Changchun, 130033, China.
Xiao MaHangzhou Branemagic Medical Technology Co. Ltd., Hangzhou, 310021, China.ORCID https://orcid.org/0009-0002-7260-9504
Kunlong ZhangHooke Laboratory, Changchun, 130033, China.
Huan WangChangchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China.
Fengzhou MaSchool of Integrated Circuits, Peking University, Beijing, 100871, China.
Qi WangCancer Translational Medicine Research Center, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116027, China.
Bei LiChangchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China.ORCID https://orcid.org/0009-0007-6702-4276
Wei WangSchool of Integrated Circuits, Peking University, Beijing, 100871, China.ORCID https://orcid.org/0000-0002-5257-7675

Funding

National Natural Science Foundation of China 62104227National Natural Science Foundation of China 82027805
6 · The paper itself

Abstract

The efficient isolation and molecular analysis of circulating tumor cells (CTCs) from whole blood at single-cell level are crucial for understanding tumor metastasis and developing personalized treatments. The viability of isolated cells is the key prerequisite for the downstream molecular analysis, especially for RNA sequencing. This study develops a laser-induced forward transfer -assisted microfiltration system (LIFT-AMFS) for high-viability CTC enrichment and retrieval from whole blood. The LIFT-compatible double-stepped microfilter (DSMF), central to this system, comprises two micropore layers: the lower layer's smaller micropores facilitate size-based cell separation, and the upper layer's larger micropores enable liquid encapsulating captured cells. By optimizing the design of the DSMFs, the system has a capture efficiency of 88% at the processing throughput of up to 15.0 mL min

Indexed as

Cell SeparationFiltrationNeoplastic Cells, CirculatingSingle-Cell AnalysisCell Line, TumorCell SurvivalHumansLaserscirculating tumor cells (CTCs)laser‐induced forward transfermicrofiltrationsingle‐cell culturesingle‐cell RNA sequencing

Identifiers

PMID39868845
PMCPMC12079465

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.