Evidence map›Paper›PMID 39883008›Full record

ArticleNucleic acids research2025

Identification of an enzyme with strong single-stranded DNA ligation activity and its application for sequencing.

Fumihito Miura, Yukiko Shibata, Miki Miura, Kazune Inatomi, Yutaka Suzuki, Takashi Ito

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Fumihito MiuraDepartment of Biochemistry, Kyushu University Graduate School of Medical Sciences, Maidashi 3-1-1, Higashi-Ku, Fukuoka 812-8582, Japan.ORCID 0000-0003-2656-486X
Yukiko ShibataDepartment of Biochemistry, Kyushu University Graduate School of Medical Sciences, Maidashi 3-1-1, Higashi-Ku, Fukuoka 812-8582, Japan.
Miki MiuraLife Science Data Research Center, Graduate School of Frontier Sciences, the University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba277-8561, Japan.
Kazune InatomiDepartment of Biochemistry, Kyushu University Graduate School of Medical Sciences, Maidashi 3-1-1, Higashi-Ku, Fukuoka 812-8582, Japan.
Yutaka SuzukiLife Science Data Research Center, Graduate School of Frontier Sciences, the University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba277-8561, Japan.
Takashi ItoDepartment of Biochemistry, Kyushu University Graduate School of Medical Sciences, Maidashi 3-1-1, Higashi-Ku, Fukuoka 812-8582, Japan.

Funding

AMED JP23ama121022JSPS JP 23H02469Research Support Project for Life Science and Drug Discovery
6 · The paper itself

Abstract

An enzyme with strong single-stranded DNA (ssDNA) ligation activity would be advantageous for many molecular biology applications. However, currently available enzymes exhibit only limited activity. Here, we identified an enzyme with strong ssDNA ligation activity upon searching the databases for proteins homologous to TS2126 RNA ligase, the known enzyme with the highest yet limited ssDNA ligation activity. A ligase from Thermus phage phiLo (GenBank: AYJ73970) or Tph ssDNA ligase (SDL) depicted higher ssDNA ligation activity than TS2126 RNA ligase. This study suggests that SDL could be useful for various applications including sequencing for library preparation.

Indexed as

DNA LigasesDNA, Single-StrandedRNA Ligase (ATP)BacteriophagesSequence Analysis, DNAThermusViral ProteinsDNA LigasesDNA, Single-StrandedRNA Ligase (ATP)Viral Proteins

Identifiers

PMID39883008
PMCPMC11780859

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.