ArticleFood chemistry2025
Development of a nanobody-based immunoassay for the detection of Escherichia coli O157:H7 in food samples.
Article in Food chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed.
- Ultrasensitive Detection ofACS omega · 2026Article
- Conformation-sensitive nanobody immunoassay enables differentiation of live and heat-inactivated Salmonella Typhimurium for food safety monitoring.Food chemistry · 2026Article
- Advances in Immunological Methods for the Detection ofSensors (Basel, Switzerland) · 2026Review
- Analytical Performance of Nanobody-Based Immunoassay and Immunosensing Platforms for Bacteria and Toxin Detection: A Systematic Review.Antibodies (Basel, Switzerland) · 2026Review
- Emerging Nanomaterials-Based Biosensors for the Clinical and Pathological Detection of E. Coli O157:H7.Current microbiology · 2026Review
- SpyTagged Mimotope Peptide Mediated Competitive Antigen-Based Rapid Quantitative Immunoassays for Uniconazole Residue.Foods (Basel, Switzerland) · 2025Article
- Development of Immunoassays for Foodborne Pathogenic Bacteria Detection Using PolyHRP for Signal Enhancement.Biosensors · 2025Article
- SERS-Based Immunochromatographic Assay for Sensitive Detection ofSensors (Basel, Switzerland) · 2025Article
- Review
- Nanobodies in animal infectious disease control: diagnosis and therapy.Frontiers in cellular and infection microbiology · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Rapid and accurate detection of foodborne pathogens is crucial for food safety. E. coli O157:H7, a major foodborne pathogen, can cause severe illness even at low concentration, highlighting the necessity for effective detection methods. This study focuses on developing nanobodies specific to E. coli O157:H7, aiming to provide a more efficient, cost-effective, and stable analytical reagents which can be available internationally. An alpaca was immunized and a phage-displayed nanobody library was constructed. Following panning, nanobodies were expressed and characterized for their affinity and specificity. A nanobody-based immunoassay was developed, with a limit of detection of 8.7 × 10
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.