Evidence map›Paper›PMID 39889709›Full record

ArticleStem cell reports2025

Effect of Notch1 signaling on muscle engraftment and maturation from pluripotent stem cells.

Aline M S Yamashita, Bayardo I Garay, Hyunkee Kim, Darko Bosnakovski, Juan E Abrahante, Karim Azzag, Phablo Abreu, Aaron Ahlquist, Rita C R Perlingeiro

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Muscle-specific increased expression ofProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Aline M S YamashitaLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Bayardo I GarayLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Hyunkee KimLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Darko BosnakovskiLillehei Heart Institute, Department of Pediatrics, University of Minnesota, Minneapolis, MN, USA.
Juan E AbrahanteUniversity of Minnesota Informatics Institute, Minneapolis, MN, USA.
Karim AzzagLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Phablo AbreuLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Aaron AhlquistLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Rita C R PerlingeiroLillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN, USA; Stem Cell Institute, University of Minnesota, Minneapolis, MN, USA. Electronic address: perli032@umn.edu.

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008244 · UNIVERSITY OF MINNESOTA TWIN CITIES · 1988 to 2005
$1.9M
Targeting Dystroglycanopathies using Pluripotent-derived Myogenic ProgenitorsR01AR081882 · NIAMS · UNIVERSITY OF MINNESOTA · 2023 to 2025
$1.0M
Preclinical studies of pluripotent stem cell-derived myogenic progenitors in non-human primatesR01AR078624 · UNIVERSITY OF MINNESOTA · 2025 to 2025
$510k
Skeletal Muscle Regeneration from Pluripotent Stem CellsR01AR078571 · UNIVERSITY OF MINNESOTA · 2025 to 2025
$409k
To explore the molecular and cellular effects of transient DUX4 expression in skeletal muscleR01AR081228 · UNIVERSITY OF MINNESOTA · 2025 to 2025
$307k
NHLBI NIH HHS F30 HL151138NIAMS NIH HHS R01 AR078571NIAMS NIH HHS R01 AR078624NIAMS NIH HHS R01 AR081228NIAMS NIH HHS R01 AR081882NIAMS NIH HHS R56 AR055299NIGMS NIH HHS T32 GM008244
6 · The paper itself

Abstract

Pax3-induced pluripotent stem cell-derived myogenic progenitors display an embryonic molecular signature but become postnatal upon transplantation. Because this correlates with upregulation of Notch signaling, here we probed whether NOTCH1 is required for in vivo maturation by performing gain- and loss-of-function studies in inducible Pax3 (iPax3) myogenic progenitors. Transplantation studies revealed that Notch1 signaling did not change the number of donor-derived fibers; however, the NOTCH1 overexpression cohorts showed enhanced satellite cell engraftment and more mature fibers, as indicated by fewer fibers expressing the embryonic myosin heavy-chain isoform and more type IIX fibers. While donor-derived Pax7+ cells were detected in all transplants, in the absence of Notch1, secondary grafts exhibited a high fraction of these cells in the interstitial space, indicating that NOTCH1 is required for proper satellite cell homing. Transcriptional profiling of NOTCH1-modified donor-derived satellite cells suggests that this may be due to changes in the extracellular matrix organization, cell cycle, and metabolism.

Indexed as

Induced Pluripotent Stem CellsPluripotent Stem CellsReceptor, Notch1Signal TransductionAnimalsCell DifferentiationMiceMuscle DevelopmentSatellite Cells, Skeletal MuscleNotch1 protein, mouseReceptor, Notch1fiber typemuscle regenerationmyogenic progenitorsNOTCH1Pax3pluripotent stem cellssatellite cellssecondary transplantation

Identifiers

PMID39889709
PMCPMC11864150

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.