Evidence map›Paper›PMID 39890843›Full record

ArticleNature communications2025

Single-cell profiling of penta- and tetradactyl mouse limb buds identifies mesenchymal progenitors controlling digit numbers and identities.

Victorio Palacio, Anna Pancho, Angela Morabito, Jonas Malkmus, Zhisong He, Geoffrey Soussi, Rolf Zeller, Barbara Treutlein, Aimée Zuniga

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Dual Bmp-negative feedback loops modulate function of both AER and ZPA to buffer and constrain postaxial digit number.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Victorio Palacio *Developmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.ORCID http://orcid.org/0009-0001-8938-5378
Anna Pancho *Developmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.ORCID http://orcid.org/0000-0002-3372-6233
Angela MorabitoDevelopmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.ORCID http://orcid.org/0009-0007-5956-6454
Jonas MalkmusDevelopmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.ORCID http://orcid.org/0009-0000-9692-8505
Zhisong HeDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.ORCID http://orcid.org/0000-0002-1502-4801
Geoffrey SoussiDevelopmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.
Rolf ZellerDevelopmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland.ORCID http://orcid.org/0000-0002-3186-7403
Barbara TreutleinDepartment of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland.ORCID http://orcid.org/0000-0002-3299-5597
Aimée ZunigaDevelopmental Genetics, Department of Biomedicine, University of Basel, Basel, Switzerland. aimee.zuniga@unibas.ch.ORCID http://orcid.org/0000-0002-9953-3637

Funding

EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) 695032Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) 310030_166685BSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) 310030_184734Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) 310030_207824
6 · The paper itself

Abstract

The cellular interactions controlling digit numbers and identities have remained largely elusive. Here, we leverage the anterior digit and identity loss in Grem1 tetradactyl mouse limb buds to identify early specified limb bud mesenchymal progenitor (LMP) populations whose size and distribution is governed by spatial modulation of BMP activity and SHH signaling. Distal-autopodial LMPs (dLMP) express signature genes required for autopod and digit development, and alterations affecting the dLMP population size prefigure the changes in digit numbers that characterize specific congenital malformations. A second, peripheral LMP (pLMP) population is anteriorly biased and reduction/loss of its asymmetric distribution underlies the loss of middle digit asymmetry and identities in Grem1 tetradactyl and pig limb buds. pLMPs depend on BMP activity, while dLMPs require GREM1-mediated BMP antagonism. Taken together, the spatial alterations in GREM1 antagonism in mouse mutant and evolutionarily diversified pig limb buds tunes BMP activity, which impacts dLMP and pLMP populations in an opposing manner.

Indexed as

Intercellular Signaling Peptides and ProteinsLimb BudsMesenchymal Stem CellsAnimalsBone Morphogenetic ProteinsCytokinesGene Expression Regulation, DevelopmentalHedgehog ProteinsMiceSignal TransductionSingle-Cell AnalysisBone Morphogenetic ProteinsCytokinesGrem1 protein, mouseHedgehog ProteinsIntercellular Signaling Peptides and ProteinsShh protein, mouse

Identifiers

PMID39890843
PMCPMC11785988

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.