Evidence map›Paper›PMID 39896297›Full record

ArticleCurrent developments in nutrition2025

Berberine Inhibits Breast Cancer Stem Cell Development and Decreases Inflammation: Involvement of miRNAs and IL-6.

Nour Ibrahim, Nawal Alsadi, Hamed Yasavoli-Sharahi, Roghayeh Shahbazi, Mary Joe Hebbo, Darshan Kambli, Florencia Balcells, Chantal Matar

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PubMed Full text at PMC Publisher
In one paragraph

Article in Current developments in nutrition, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nour IbrahimNutritional Sciences Department, Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario, Canada.
Nawal AlsadiCellular and Molecular in Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Hamed Yasavoli-SharahiCellular and Molecular in Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Roghayeh ShahbaziCellular and Molecular in Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Mary Joe HebboNutritional Sciences Department, Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario, Canada.
Darshan KambliCellular and Molecular in Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Florencia BalcellsCellular and Molecular in Medicine Department, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Chantal MatarNutritional Sciences Department, Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Breast cancer (BC) is a health concern worldwide and is often accompanied by depressive symptoms in patients. In BC, elevated interleukin-6 (IL-6) levels contribute to an inflammatory signature linked to cancer stem cell (CSC) stemness and depressive behaviors. Bioactive food components, such as berberine (BBR), have preventative effects against BC by targeting CSCs. Objectives: This study aimed to investigate the effects of BBR on breast CSC proliferation, on levels of specific micro (mi)RNAs and IL-6 in vitro and in vivo, and in alleviating depressive-like behaviors in mice with BC. Methods: Mammosphere formation assays were conducted by treating murine 4T1 and human MDA-MB-231 BC cell lines with BBR. qPCR analysis of miRNAs miR-let-7c and miR-34a-5p was performed on 4T1 CSCs exposed to BBR. BBR was administered orally to female BALB/c, followed by injection with mammary carcinoma cells to induce BC. Behavioral tests were conducted to assess depressive-like behaviors. Tumor tissues were collected for ex vivo mammosphere assays, miRNA expression analysis, and IL-6 detection by ELISA. Serum was also collected for IL-6 analysis. Results: BBR treatment inhibited mammosphere formation and proliferation of CSCs derived from 4T1 and MDA-MB-231 cell lines. Quantification of mammosphere formation showed a significant decrease in both cell lines at 75 μM BBR (4T1: Conclusions: BBR may have the potential to be used as an "Epi-Natural Compound" to prevent cancer by reducing inflammation and affecting epigenetics.

Indexed as

berberinebreast cancercancer stem cellsepigeneticsinterleukin-6microRNAs

Identifiers

PMID39896297
PMCPMC11786844

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.