Evidence map›Paper›PMID 39905036›Full record

ArticleScientific reports2025

Production of novel theranostic nano-vector based on superparamagnetic iron oxide nanoparticles/miR-497 targeting colorectal cancer.

Asmaa M Elfiky, May M Eid, May El-Manawaty, Zeinab A Elshahid, Elham Mohamed Youssef, Khaled Mahmoud

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Asmaa M Elfiky *Environmental and Occupational Medicine Department, Environment and Climate Change Research Institute, National Research Centre, Cairo, Egypt. am.elfiky@nrc.sci.eg.
May M Eid *Physics Institute, National Research Center, Dokki, Cairo, Egypt.
May El-ManawatyPharmaceutical Sciences Institute, Department of Pharmacognosy, National Research Centre, Cairo, Egypt.
Zeinab A Elshahid *Chemistry of Natural and Microbial Products, Pharmaceutical Industry Research Institute, National Research Centre, Cairo, Egypt.
Elham Mohamed YoussefBiochemistry Department, National Research Centre, Cairo, Egypt.
Khaled MahmoudPharmaceutical Sciences Institute, Department of Pharmacognosy, National Research Centre, Cairo, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colorectal cancer (CRC) is a serious public health concern worldwide. Immune checkpoint inhibition medication is likely to remain a crucial part of CRC clinical management. This study aims to create new super paramagnetic iron oxide nano-carrier (SPION) that can effectively transport miRNA to specific CRC cell lines. In addition, evaluate the efficiency of this nano-formulation as a therapeutic candidate for CRC. Bioinformatics tools were used to select a promising tumor suppressor miRNA (mir-497-5p). Green route, using Fusarium oxyporium fungal species, manipulated for the synthesis of SPION@Ag@Cs nanocomposite as a carrier of miR-497-5p. That specifically targets the suppression of PD1/PDL1 and CTLA4pathways for colorectal therapy. UV/visible and FTIR spectroscopy, Zeta potential and MTT were used to confirm the allocation of the miR-497 on SPION@Ag@Cs and its cytotoxicity against CRC cell lines. Immunofluorescence was employed to confirm transfection of cells with miR-497@NPs, and the down- regulation of CTLA4 in HT29, and Caco2 cell lines. On the other hand, PDL1 showed a significant increase in colorectal cell lines (HT-29 and Caco-2) in response to mir497-5p@Nano treatment. The data suggest that the mir-497 -loaded SPION@Ag@Cs nano-formulation could be a good candidate for the suppression of CTLA4in CRC human cell lines. Consequently, the targeting miR-497/CTLA4 axis is a potential immunotherapy treatment strategy for CRC.

Indexed as

Colorectal NeoplasmsMagnetic Iron Oxide NanoparticlesMicroRNAsTheranostic NanomedicineB7-H1 AntigenCaco-2 CellsCell Line, TumorCTLA-4 AntigenHT29 CellsHumansB7-H1 AntigenCD274 protein, humanCTLA-4 AntigenCTLA4 protein, humanMicroRNAsMIRN497 microRNA, humanColorectal cancerCTLA-4 oncogenesmiR-497-5pPD1/PDL1SPION@Ag@Cs nano-carrier

Identifiers

PMID39905036
PMCPMC11794539

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.