Evidence map›Paper›PMID 39908322›Full record

ArticlePLoS genetics2025

Plant-specific cochaperone SSR1 affects root elongation by modulating the mitochondrial iron-sulfur cluster assembly machinery.

Xuanjun Feng, Yue Hu, Tao Xie, Huiling Han, Diana Bonea, Lijuan Zeng, Jie Liu, Wenhan Ying, Bona Mu, Yuanyuan Cai and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS genetics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xuanjun FengState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Sichuan, China.ORCID https://orcid.org/0000-0002-3800-4029
Yue HuMaize Research Institute of Sichuan Agricultural University, Wenjiang, Sichuan, China.
Tao XieKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
Huiling HanKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
Diana BoneaDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada.
Lijuan ZengSichuan Keyuan Testing Center of Engineering Technology Co., Ltd; Chengdu, Sichuan, PR China.
Jie LiuKey Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing, China.ORCID https://orcid.org/0009-0006-2524-2278
Wenhan YingKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.
Bona MuDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada.
Yuanyuan CaiKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.ORCID https://orcid.org/0009-0004-8231-2709
Min ZhangInstitute of Genetics and Developmental Biology, Beijing, China.
Yanli LuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Sichuan, China.
Rongmin ZhaoDepartment of Biological Sciences, University of Toronto Scarborough, Toronto, Ontario, Canada.ORCID https://orcid.org/0000-0003-1300-5882
Xuejun HuaKey Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, Zhejiang, China.ORCID https://orcid.org/0000-0002-9055-8416

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To elucidate the molecular function of SHORT AND SWOLLEN ROOT1 (SSR1), we screened for suppressors of the ssr1-2 (sus) was performed and identified over a dozen candidates with varying degrees of root growth restoration. Among these, the two most effective suppressors, sus1 and sus2, resulted from G87D and T55M single amino acid substitutions in HSCA2 (At5g09590) and ISU1 (At4g22220), both crucial components of the mitochondrial iron-sulfur (Fe-S) cluster assembly machinery. SSR1 displayed a robust cochaperone-like activity and interacted with HSCA2 and ISU1, facilitating the binding of HSCA2 to ISU1. In comparison to the wild-type plants, ssr1-2 mutants displayed increased iron accumulation in root tips and altered expression of genes responsive to iron deficiency. Additionally, the enzymatic activities of several iron-sulfur proteins and the mitochondrial membrane potential were reduced in ssr1-2 mutants. Interestingly, SSR1 appears to be exclusive to plant lineages and is induced by environmental stresses. Although HSCA2G87D and ISU1T55M can effectively compensate for the phenotypes associated with SSR1 deficiency under favorable conditions, their compensatory effects are significantly diminished under stress. Collectively, SSR1 represents a new and significant component of the mitochondrial Fe-S cluster assembly (ISC) machinery. It may also confer adaptive advantages on plant ISC machinery in response to environmental stress.

Indexed as

ArabidopsisArabidopsis ProteinsIron-Sulfur ProteinsMolecular ChaperonesPlant RootsGene Expression Regulation, PlantIronMitochondriaMitochondrial ProteinsMutationSulfurArabidopsis ProteinsIronIron-Sulfur ProteinsMitochondrial ProteinsMolecular ChaperonesSulfur

Identifiers

PMID39908322
PMCPMC11835332

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.