Evidence map›Paper›PMID 39916610›Full record

ArticleDisease models & mechanisms2025

The inflammasome adaptor pycard is essential for immunity against Mycobacterium marinum infection in adult zebrafish.

Meri Uusi-Mäkelä, Sanna-Kaisa Emilia Harjula, Maiju Junno, Alina Sillanpää, Reetta Nätkin, Mirja Tellervo Niskanen, Anni Karoliina Saralahti, Matti Nykter, Mika Rämet

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Disease models & mechanisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Meri Uusi-MäkeläFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Sanna-Kaisa Emilia HarjulaFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Maiju JunnoFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Alina SillanpääFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Reetta NätkinProstate Cancer Research Center, Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Mirja Tellervo NiskanenFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Anni Karoliina SaralahtiFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Matti NykterProstate Cancer Research Center, Faculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.
Mika RämetFaculty of Medicine and Health Technology, Tampere University, FI-33014 Tampere, Finland.ORCID 0000-0002-5776-164X

Funding

Competitive State Research Financing of the Expert Responsibility Area of Oulun Yliopistollinen SairaalaFinnish Society for Study of Infectious DiseasesInstrumentariumin TiedesäätiöMaud Kuistilan MuistosaatioMaud Kuistilan MuistosäätiöNational Doctoral Education Pilot Based on the Immune SystemOrion Research FoundationResearch Council of Finland 277495Sigrid Juséliuksen SäätiöSuomen KulttuurirahastoTampereen TuberkuloosisaatioTampereen TuberkuloosisäätiöTampereen YliopistoTampere University Doctoral SchoolThe Academy of Finland 277495The Competitive State Research Financing of the Expert Responsibility Area of Oulu University HospitalVäinö ja Laina Kiven Säätiö
6 · The paper itself

Abstract

Inflammasomes regulate the host response to intracellular pathogens including mycobacteria. We have previously shown that the course of Mycobacterium marinum infection in adult zebrafish (Danio rerio) mimics the course of tuberculosis in human. To investigate the role of the inflammasome adaptor pycard in zebrafish M. marinum infection, we produced two zebrafish knockout mutant lines for the pycard gene with CRISPR/Cas9 mutagenesis. Although the zebrafish larvae lacking pycard developed normally and had unaltered resistance against M. marinum, the loss of pycard led to impaired survival and increased bacterial burden in the adult zebrafish. Based on histology, immune cell aggregates, granulomas, were larger in pycard-deficient fish than in wild-type controls. Transcriptome analysis with RNA sequencing of a zebrafish haematopoietic tissue, kidney, suggested a role for pycard in neutrophil-mediated defence, haematopoiesis and myelopoiesis during infection. Transcriptome analysis of fluorescently labelled, pycard-deficient kidney neutrophils identified genes that are associated with compromised resistance, supporting the importance of pycard for neutrophil-mediated immunity against M. marinum. Our results indicate that pycard is essential for resistance against mycobacteria in adult zebrafish.

Indexed as

CARD Signaling Adaptor ProteinsImmunityInflammasomesMycobacterium Infections, NontuberculousMycobacterium marinumZebrafishZebrafish ProteinsAnimalsCRISPR-Cas SystemsGene Expression ProfilingGene Knockout TechniquesGranulomaKidneyLarvaNeutrophilsTranscriptomeCARD Signaling Adaptor ProteinsInflammasomesZebrafish ProteinsInflammasomeMycobacterium marinumPycardTuberculosisZebrafish

Identifiers

PMID39916610
PMCPMC11972081

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.