Evidence map›Paper›PMID 39925828›Full record

ArticleIranian journal of veterinary research2024

The first comprehensive study on isolation and genetic characterization of canine parvoviruses from dogs in Mizoram, India reveals the emergence of CPV-2c.

P Chakraborty, S K Behera, M H Lalhriatchhungi, P Roychoudhury, L Maibam, P Behera, J K Chaudhary, H Prasad, J B Rajesh, M Lalhmangaihzuali and 5 more

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Article in Iranian journal of veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

P ChakrabortyPh.D. Student in Veterinary Medicine, Department of Veterinary Medicine, West Bengal University of Animal and Fishery Sciences, West Bengal, Kolkata-700037, India.
S K BeheraDepartment of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
M H LalhriatchhungiGraduated from Regional Institute of Paramedical and Nursing Sciences, Zemabawk-796 017, Aizawl, Mizoram, India.
P RoychoudhuryDepartment of Veterinary Microbiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
L MaibamMVSc in Veterinary Medicine, Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
P BeheraDepartment of Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
J K ChaudharyDepartment of Animal Breeding and Genetics, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
H PrasadDepartment of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
J B RajeshDepartment of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
M LalhmangaihzualiMVSc in Veterinary Medicine, Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
H C J MaryMVSc in Veterinary Medicine, Department of Veterinary Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
L H LalrosangaMVSc in Veterinary Surgery and Radiology, Department of Veterinary Surgery and Radiology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
M S DawnglianaMVSc in Animal Reproduction and Gynaecology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih-796 015, Aizawl, Mizoram, India.
V ChanderDivision of Immunology (CADRAD), ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India.
M KumarDepartment of Veterinary Medicine, Bihar Veterinary College, Bihar Animal Sciences University, Patna-800014, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Canine parvovirus type-2 (CPV-2) is a highly contagious enteric pathogen of puppies with worldwide distribution. Aims: Molecular epidemiology, genetic characterization, phylogenetic analysis, and isolation of the CPV-2 virus from clinically affected dogs in Mizoram, India over eight years. Methods: A total of 202 samples (199 fecal samples, 2 vomita, and 1 tissue sample) were screened by PCR assay. Results: 103 out of 202 samples (50.99%) tested positive. Of the 103 positive samples, 83 samples were cloned and sequenced. Sequence analysis showed CPV-2c as the predominant variant (63.85%) followed by the 2a variant (26.5%), 2b (8.43%), and FPV (1.2%). Phylogenetic analyses of the CPV-2c sequences formed separate clusters and were ancestrally related to Japanese, Chinese, and Italian 2c sequences. Similarly, 2a isolates formed separate clusters under different clades and were ancestrally related to Indian, Singaporean, Japanese, Uruguayan, and Chinese 2a isolates. 2b isolates formed a single cluster with the Chinese 2b isolate. FPV isolate clustered with North American FPV. Both synonymous and non-synonymous mutations (unique to this study) were evident in all the types of CPV-2s indicative of active evolution with regional variation. In the cell culture medium, CPV-2 showed cytopathogenic effects at the third passage level. Conclusion: The study, the first in-depth report on CPV-2, showed a shift towards CPV-2c as the predominant variant in Mizoram. This variant clustered separately from current vaccine strains, highlighting the need for extensive epidemiological surveillance to better understand viral phylogenomics and evaluate current vaccine efficacy.

Indexed as

CPV-2cEpidemiologyIsolationMizoramMutations

Identifiers

PMID39925828
PMCPMC11801323

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.