Evidence map›Paper›PMID 39927486›Full record

ArticleFEBS open bio2025

Acacetin reduces endoplasmic reticulum stress through the P-eNOS/PERK signaling pathway to attenuate MGO-induced vascular endothelial cell dysfunction.

Zhen Zhang, Kaien Hu, Zhaohui Fang, Sihai Wang, Jie Chen, Dengke Yin, Caiyun Zhang, Gefei Ma

Abstract read
In one paragraph

Article in FEBS open bio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhen ZhangSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Kaien HuSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Zhaohui FangDepartment of Endocrine, The First Hospital Affiliated to Anhui University of Chinese Medicine, Hefei, Anhui, China.
Sihai WangDepartment of Endocrine, The First Hospital Affiliated to Anhui University of Chinese Medicine, Hefei, Anhui, China.
Jie ChenSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Dengke YinSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Caiyun ZhangSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.
Gefei MaSchool of Pharmacy, Anhui University of Chinese Medicine, Hefei, Anhui, China.ORCID https://orcid.org/0009-0000-1962-9616

Funding

National Natural Science Foundation of China No. 82174153
6 · The paper itself

Abstract

Diabetic macrovascular disease is one of the most morbid and deadly complications of diabetes. Endothelial dysfunction plays a key role in diabetic macrovascular complications and endothelial cell apoptosis is one of the key indicators of endothelial dysfunction. Methylglyoxal (MGO), a highly reactive dicarbonyl compound generated during glycolysis, is related to the pathogenesis of cardiovascular diseases and may also promote endothelial dysfunction. Acacetin (ACA) is a naturally occurring flavonoid that can inhibit apoptosis, oxidative stress and inflammation to slow the progression of coronary heart disease; however, its effects on endothelial dysfunction are unknown. The present study investigated whether ACA may ameliorate MGO-induced endothelial dysfunction in human umbilical vein endothelial cells. The results revealed that the viability and apoptosis of human umbilical vein endothelial cells induced by MGO decreased after ACA treatment, which was reflected in the expression levels of the apoptosis-related proteins b-cell lymphoma 2 (Bcl-2)-associated death, Bcl-2-associated x protein and Bcl-2. Additionally, ACA downregulated the expression of key protein markers of MGO-induced endoplasmic reticulum stress, physical evidence recovery kit, eukaryotic initiation factor 2 alpha, activating transcription factor 4 and C/EBP homologous protein, with which calcium inward currents may be closely related. ACA significantly downregulated the MGO-induced expression of the cytosolic calcium channel proteins stromal interaction molecule 1, transient receptor potential canonical 1, ORAI calcium release-activated calcium modulator 1, transient receptor potential vanilloid 1 and 4, and the trans-endoplasmic reticulum membrane protein, transmembrane and coiled-coil domains 1. Finally, ACA increased the expression of phosphorylated endothelial nitric oxide synthase (Ser1177), thus increasing the expression of nitric oxide in endothelial cells. Overall, acacetin could reduce endoplasmic reticulum stress through the phosphorylated-endothelial nitric oxide/physical evidence recovery kit signaling pathway to attenuate MGO-induced vascular endothelial cell dysfunction. These findings may hold potential for the use of acacetin in diabetic macrovascular complications.

Indexed as

Endoplasmic Reticulum StressFlavonesApoptosiseIF-2 KinaseHumansHuman Umbilical Vein Endothelial CellsNitric Oxide Synthase Type IIIOxidative StressPyruvaldehydeSignal TransductionacacetineIF-2 KinaseFlavonesNitric Oxide Synthase Type IIINOS3 protein, humanPyruvaldehydeacacetincalcium homeostasisdiabetic vascular diseasesendoplasmic reticulum stressendothelial dysfunctionHUVECs apoptosis

Identifiers

PMID39927486
PMCPMC12051029

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.