Evidence map›Paper›PMID 39928721›Full record

ReviewChemical reviews2025

Optogenetics with Atomic Precision─A Comprehensive Review of Optical Control of Protein Function through Genetic Code Expansion.

Maura Charette, Carolyn Rosenblum, Olivia Shade, Alexander Deiters

Abstract readReview
In one paragraph

Review in Chemical reviews, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Precise Regulation of Membrane Proteins: From Physical Technology to Biomolecular Strategy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Maura CharetteDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.ORCID 0009-0002-7186-4773
Carolyn RosenblumDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
Olivia ShadeDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.
Alexander DeitersDepartment of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States.ORCID 0000-0003-0234-9209

Funding

"On the Fly" Time Resolved Cryo-EM Studies of Intermediate HIV-1 RT Transition StatesR01AI175067 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Guillermo Alberto Calero, Alexander Deiters · 2022 to 2026
$3.8M
Principles for Designing Stimulus-Responsive EnzymesR01GM132565 · NIGMS · RUTGERS, THE STATE UNIV OF N.J. · PI KHARE, SAGAR D · 2019 to 2022
$1.8M
NIAID NIH HHS R01 AI175067NIGMS NIH HHS R01 GM132565
6 · The paper itself

Abstract

Conditional control of protein activity is important in order to elucidate the particular functions and interactions of proteins, their regulators, and their substrates, as well as their impact on the behavior of a cell or organism. Optical control provides a perhaps optimal means of introducing spatiotemporal control over protein function as it allows for tunable, rapid, and noninvasive activation of protein activity in its native environment. One method of introducing optical control over protein activity is through the introduction of photocaged and photoswitchable noncanonical amino acids (ncAAs) through genetic code expansion in cells and animals. Genetic incorporation of photoactive ncAAs at key residues in a protein provides a tool for optical activation, or sometimes deactivation, of protein activity. Importantly, the incorporation site can typically be rationally selected based on structural, mechanistic, or computational information. In this review, we comprehensively summarize the applications of photocaged lysine, tyrosine, cysteine, serine, histidine, glutamate, and aspartate derivatives, as well as photoswitchable phenylalanine analogues. The extensive and diverse list of proteins that have been placed under optical control demonstrates the broad applicability of this methodology.

Indexed as

Amino AcidsGenetic CodeOptogeneticsProteinsAnimalsHumansAmino AcidsProteins

Identifiers

PMID39928721
PMCPMC11869211

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.