Melatonin Mitigates Acidosis-Induced Neuronal Damage by Up-Regulating Autophagy via the Transcription Factor EB.

Yan Shi, Zhaoyu Mi, Wei Zhao, Yue Hu, Hui Xiang, Yaoxue Gan, Shishan Yuan

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yan ShiKey Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Pharamceutical Sciences, Health Science Center, Hunan Normal University, Changsha 410013, China.

Zhaoyu MiSchool of Medical Technology and Translational Medicine, Hunan Normal University, Changsha 410006, China.

Wei ZhaoSchool of Medical Technology and Translational Medicine, Hunan Normal University, Changsha 410006, China.

Yue HuSchool of Medical Technology and Translational Medicine, Hunan Normal University, Changsha 410006, China.

Hui XiangSchool of Medical Technology and Translational Medicine, Hunan Normal University, Changsha 410006, China.

Yaoxue GanSchool of Medical Technology and Translational Medicine, Hunan Normal University, Changsha 410006, China.

Shishan YuanKey Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Pharamceutical Sciences, Health Science Center, Hunan Normal University, Changsha 410013, China.

Funding

Hunan Province Developmental Biology and Biological Breeding Key Programs 2022XKQ0205Natural Science Foundation of China U22A20303Scientific Research Project of Hunan Provincial Health Commission D202302089587

6 · The paper itself

Abstract

Acidosis, a common feature of cerebral ischemia and hypoxia, results in neuronal damage and death. This study aimed to investigate the protective effects and mechanisms of action of melatonin against acidosis-induced neuronal damage. SH-SY5Y cells were exposed to an acidic environment to simulate acidosis, and a photothrombotic (PT) infarction model was used to establish an animal model of cerebral ischemia of male C57/BL6J mice. Both in vivo and in vitro studies demonstrated that acidosis increased cytoplasmic transcription factor EB (TFEB) levels, reduced nuclear TFEB levels, and suppressed autophagy, as evidenced by elevated p62 levels, a higher LC3-II/LC3-I ratio, decreased synapse-associated proteins (PSD-95 and synaptophysin), and increased neuronal apoptosis. In contrast, melatonin promoted the nuclear translocation of TFEB, enhanced autophagy, and reversed neuronal apoptosis. Moreover, the role of TFEB in melatonin's neuroprotective effects was validated by modulating TFEB nuclear translocation. In conclusion, melatonin mitigates acidosis-induced neuronal damage by promoting the nuclear translocation of TFEB, thereby enhancing autophagy. These findings offer new insights into potential treatments for acidosis.

Indexed as

AcidosisAutophagyBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsMelatoninNeuronsNeuroprotective AgentsAnimalsApoptosisBrain IschemiaCell Line, TumorDisease Models, AnimalHumansMaleMiceMice, Inbred C57BLUp-RegulationBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsMelatoninNeuroprotective AgentsTcfeb protein, mouseTFEB protein, humanacidosisautophagymelatoninneuroprotectiveTFEB

Identifiers

PMID39940940

DOI10.3390/ijms26031170

PMCPMC11818126

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.