Transgressive expression and dosage effect of A09 chromosome genes and their homoeologous genes influence the flowering time of resynthesized allopolyploid Brassica napus.

backgroundThe genomes of allopolyploids newly formed through hybridization and polyploidization exhibit substantial changes including those at genetic and epigenetic levels. These alterations may affect their gene expression patterns, leading to nonadditive gene expression. Currently, only a few reports are available on the impact of nonadditive gene expressions on traits.

resultsUsing six isogenic resynthesized Brassica napus lines across the first 10 generations, we studied the impact of gene expression patterns on flowering time. The expression levels of a group of genes, located on chromosome A09, were significantly positively correlated with flowering time. According to the expression analysis, the expression levels of the homologous pairs of 139 genes on chromosome A09 were lower in allopolyploids than in their diploid parents, which indicated a phenomenon of transgressive expression. Additionally, independent subgenomic analysis of homoeologous gene pairs on chromosome A09 of the allopolyploids demonstrated that the gene expression levels of B. napus subgenome A (BnA) and subgenome C (BnC) were similar. However, in two aneuploids carrying monosomic or trisomic A09 chromosome, the gene expression levels of BnA were lower or higher than those of BnC, and the corresponding flowering times of these two aneuploids were earlier and later, respectively.

conclusionsThese findings indicate that changes in gene dosage introduce biases in the expression of homoeologous genes. Moreover, upregulation or downregulation of homoeologous gene expression on a single chromosome partially alters the flowering time of the newly formed allopolyploid B. napus, which is of great significance for horticultural applications and future research on genetic mechanisms.