ReviewJournal of biophotonics2025
Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.
Review in Journal of biophotonics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Development of solid-state fluorescence lifetime standards for clinical applications using dyed epoxy resins.Journal of biomedical optics · 2026Article
- More Than a Glow: How Cyanine Dyes Shape Tracer Pharmacokinetics.Angewandte Chemie (International ed. in English) · 2026Review
- Neural Network-Based System for Rapid Diagnostic Decision Support Using FLIM Data: Segmentation and Classification in Liver, Skin, and Pancreatic Tissues.International journal of molecular sciences · 2026Article
- Partitioning of the truncated insulin receptor DAF-2B between homodimers and heterodimers influences insulin signaling in C. elegans.PLoS genetics · 2026Article
- Article
- Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.Journal of biomedical optics · 2026Article
- Optical, Tomographic, and Mass Spectrometry Imaging Methods for Burn Wounds: Capabilities, Limitations, and Clinical Potential.Biomedicines · 2026Review
- Prediction of Patients' Response to Immune Checkpoint Inhibitors Using Fluorescence Lifetime Imaging of Lymphocytes.Biomedicines · 2026Article
- Advances in fluorescence-based point-of-care diagnostics: probes, nanostructures and integrated systems.Journal of materials chemistry. C · 2026Review
- Fluorescence properties of methylene blue conjugated to normal/cancerous human tissues for spectral discrimination of breast cancer stages (I-IV).Biomedical optics express · 2026Article
- PSF-Driven Spatio-Temporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.bioRxiv : the preprint server for biology · 2026Article
- From Population Averaging to Single Event Resolution: Evolution of Sensing Platforms for Membrane Fusion.Sensors (Basel, Switzerland) · 2026Review
- Two-Step Ligand-Directed Covalent Fluorescent Labeling of the Adenosine AJournal of medicinal chemistry · 2026Article
- Chemical fluorophores for fluorescence lifetime imaging.Chemical Society reviews · 2026Review
- Multi-parameter fluorescence lifetime imaging for high-noise neuroscience applications.Biomedical optics express · 2026Article
- Multi-rotor imidazole-based fluorophores for rapid delineation of squamous cell carcinoma infiltration margins in murine surgical specimens.Frontiers in chemistry · 2026Article
- Investigating Cellular Magnetic Bioeffects Using Two-Channel, Two-Photon Autofluorescence Lifetime Microscopy.IEEE transactions on molecular, biological, and multi-scale communications · 2026Article
- Visualizing intracellular milieu and electric field-induced cell responses by fluorescence lifetime imaging microscopy.Scientific reports · 2025Article
- Fluorescence Lifetime Imaging Techniques-A Review on Principles, Applications and Clinical Relevance.Journal of biophotonics · 2025Review
- Optical express-biopsy of gliomas using macroscopic fluorescence lifetime imaging.Biomedical optics express · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
This article gives an overview of the most frequently used fluorescence-lifetime imaging (FLIM) techniques, their capabilities, and typical applications. Starting from a general introduction to fluorescence and phosphorescence lifetime, we will show that the fluorescence lifetime or, more accurately, the fluorescence decay function of a fluorophore is a direct indicator of the interaction with its molecular environment. FLIM is therefore more than a simple contrast technique in microscopy-it is a technique of molecular imaging. FLIM techniques can be classified into time-domain and frequency-domain techniques, analogue and photon counting techniques, and scanning and wide-field techniques. Starting from an overview of these general technical principles we will describe the features and peculiarities of the different FLIM techniques in use. An extended section is dedicated to TCSPC FLIM, addressing unique capabilities that make the technique especially interesting to FLIM of biological systems.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.