In vivo and in silico studies on the potential role of garden cress oil in attenuating methotrexate-induced inflammation and apoptosis in liver.

Dalia M Mabrouk, Radwa H El-Akad, Ahmed H Afifi, Hafiza A Sharaf, Sonia L El-Sharkawy, Aida I El Makawy

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 citing paper in PubMed.

Design, Synthesis, X-Ray Crystallographic Characterization, Anticholinesterase and Antioxidant Evaluation, and Molecular Modeling of Novel Dispiroindene-Pyrrolidine Derivatives as Multifunctional Anti-Alzheimer Agents.Drug development research · 2026
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6 authors.

Dalia M MabroukCell Biology Department, Biotechnology Research Institute, National Research Centre, P.O.12622, Giza, Egypt.

Radwa H El-AkadPharmacognosy Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, PO Box 12622, Cairo, Egypt.

Ahmed H AfifiPharmacognosy Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, PO Box 12622, Cairo, Egypt.

Hafiza A SharafPathology Department, Medical Research and Clinical Studies Institute, National Research Centre, P.O.12622, Giza, Egypt.

Sonia L El-SharkawyPathology Department, Medical Research and Clinical Studies Institute, National Research Centre, P.O.12622, Giza, Egypt.

Aida I El MakawyCell Biology Department, Biotechnology Research Institute, National Research Centre, P.O.12622, Giza, Egypt. aelmakawy@yahoo.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methotrexate (MTX) has been used in high doses for cancer therapy and low doses for autoimmune diseases. It is proven that methotrexate-induced hepatotoxicity occurs even at relatively low doses. It is known that garden cress has anti-inflammatory, antioxidant, and hepatoprotective properties. This study investigates the potential alleviating effect of garden cress oil (GCO) against MTX-induced hepatotoxicity in rats. The chemical composition of GCO was assessed using GC/MS analysis. Liver damage was studied using hepatotoxicity biomarkers, molecular, and histological analysis. Also, the effects of GCO on TNF-α and caspase-3 proteins were evaluated through molecular docking studies. The results demonstrated that MTX caused liver damage, as seen by elevated levels of the liver enzymes ALT, AST, and ALP. Likewise, MTX showed clear signs of apoptosis, such as increased mRNA expression levels of BAX, Caspase-3, and P53, and increased liver inflammation indicated by higher levels of TNF-α expression. MTX exhibited significant liver damage, as demonstrated by histological examination. Treatment with GCO effectively alleviated the apoptotic effects of MTX, provided protection against inflammation, and restored histological alterations. GC/MS metabolite profiling of garden cress oil revealed the presence of several phytoconstituents, including tocopherols, erucic acid, sesamolin, linoleic acid, vaccenic acid, oleic acid, stearic acid, and palmitic acid, that showed strong binding affinities toward TNF-α and caspase-3 proteins in molecular docking studies, which could explain the anti-apoptotic and anti-inflammatory potential of GCO.

Indexed as

ApoptosisChemical and Drug Induced Liver InjuryInflammationLiverMethotrexatePlant OilsAnimalsAnti-Inflammatory AgentsCarumCaspase 3Computer SimulationGas Chromatography-Mass SpectrometryMaleMolecular Docking SimulationRatsTumor Necrosis Factor-alphaAnti-Inflammatory Agentscaraway oilCaspase 3MethotrexatePlant OilsTumor Necrosis Factor-alphaApoptosisGarden cressHepatotoxicityInflammationIn silicoMethotrexate

Identifiers

PMID39979397

DOI10.1038/s41598-025-89550-8

PMCPMC11842783

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