ArticleBMC oral health2025
Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2.
Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.
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Who cites it
4 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Role of MicroRNA in the Behaviour of Periodontal Ligament Stem Cells and Stem Cells from the Apical Papilla: A Systematic Review.Stem cell reviews and reports · 2026Pooled it
- FRZB regulates the osteogenic differentiation of periodontal ligament stem cells in an inflammatory microenvironment through Wnt5a-mitochondrial axis.Cell regeneration (London, England) · 2026Article
- Resolvin D1 Modulates the Inflammatory Processes of Human Periodontal Ligament Cells via NF-κB and MAPK Signaling Pathways.Biomedicines · 2025Article
- MiR-33 as a novel diagnostic biomarker for distinguishing cholesterol from adenomatous polyps: a case-control study.Hereditas · 2025Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPeriodontitis is a prevalent oral disease that significantly impacts human quality of life. This study aimed at the influence of microRNA-299-5p (miR-299-5p) on regulating lipopolysaccharide (LPS)-induced osteogenic differentiation of periodontal ligament stem cells (PDLSCs).
methodsThe 105 periodontitis patients and 102 healthy periodontal volunteers (HC) were recruited with their clinical baseline data. miR-299-5p expression in saliva was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). To identify the target relationship between miR-299-5p and pumilio RNA-binding family member 2 (PUM2), the dual luciferase reporter gene was explored. PDLSCs were treated with LPS and subjected to osteogenic differentiation induction. The secretion of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) was determined by enzyme-linked immunosorbent assay. mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN) was detected by qRT-PCR.
resultsmiR-299-5p expression was markedly reduced in periodontitis patients' saliva and negatively correlated with patients' attachment loss, bleeding index, plaque index, probing pocket depth, and TNF-α, IL-6, and IL-1β levels. miR-299-5p could be well distinguished between periodontitis and HC. LPS induction dramatically stimulated the secretion of inflammatory factors and inhibited the differentiation of PDLSCs, which was counteracted by miR-299-5p overexpression. miR-299-5p was detected to target PUM2, which could exacerbate the inflammatory condition of PDLSCs and lead to differentiation defects.
conclusionsmiR-299-5p has emerged as a promising diagnostic marker for periodontitis and may attenuate inflammation and contribute to osteogenic differentiation in PDLSCs cells by targeting PUM2.
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