Evidence map›Paper›PMID 39989284›Full record

ArticleThe international journal of neuropsychopharmacology2025

Altered proteomics in brain extracellular vesicles from depressed individuals who died by suicide implicates synaptic processes.

Pascal Ibrahim, Haruka Mitsuhashi, Lorne Taylor, Jenna Cleyle, Naguib Mechawar, Corina Nagy, Gustavo Turecki

Abstract read
In one paragraph

Article in The international journal of neuropsychopharmacology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Pascal IbrahimIntegrated Program in Neuroscience, McGill University, Montreal, Quebec, Canada.
Haruka MitsuhashiIntegrated Program in Neuroscience, McGill University, Montreal, Quebec, Canada.
Lorne TaylorProteomics and Molecular Analysis Platform, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
Jenna CleyleProteomics and Molecular Analysis Platform, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
Naguib MechawarIntegrated Program in Neuroscience, McGill University, Montreal, Quebec, Canada.
Corina NagyIntegrated Program in Neuroscience, McGill University, Montreal, Quebec, Canada.ORCID 0000-0003-1439-0129
Gustavo TureckiIntegrated Program in Neuroscience, McGill University, Montreal, Quebec, Canada.ORCID 0000-0003-4075-2736

Funding

Canadian Institute of Health Research (CIHR) ENP161427Canadian Institute of Health Research (CIHR) FDN148374
6 · The paper itself

Abstract

backgroundMajor depressive disorder (MDD) is a common and debilitating disorder whose molecular neurobiology remains unclear. Extracellular vesicles (EVs) are small vesicles that are released by cells and are involved in intercellular communication. They carry bioactive molecules, such as proteins, that reflect the state of their cell of origin. In this study, we sought to investigate the proteomic cargo of brain EVs from depressed individuals as compared to EVs from matched neurotypical individuals. In addition, we investigated how the EV proteomic cargo compares to the proteomic profile of bulk tissue.

methodsUsing mass spectrometry and label-free quantification, we investigated the EV and bulk tissue protein profile from anterior cingulate cortex samples from 86 individuals. We performed differential expression analysis to compare cases and controls, followed by in silico analysis to determine potential implicated functions of dysregulated proteins.

resultsExtracellular vesicles display distinct proteomic profiles compared to bulk tissue. Differential expression analysis showed that 70 proteins were differentially packaged in EVs in MDD, while there was no significant difference in protein levels between groups in bulk tissue. In silico analysis points to a strong role of these differential EV proteins in synaptic functions.

conclusionTo our knowledge, this is the first study to profile EV proteins in depression, providing novel information to better understand the pathophysiology of MDD. This work paves the way for discovering new therapeutic targets for MDD and prompts more investigations into EVs in MDD and other psychiatric disorders.

Indexed as

Extracellular VesiclesGyrus CinguliMajor Depressive DisorderProteomeSuicide, CompletedSynapsesAdultFemaleHumansMaleMiddle AgedProteomicsProteomedepressionextracellular vesiclesLC-MS/MSpostmortemproteomics

Identifiers

PMID39989284
PMCPMC12122421

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.