Current Paradigms and Future Challenges in Harnessing Nanocellulose for Advanced Applications in Tissue Engineering: A Critical State-of-the-Art Review for Biomedicine.

Nanocellulose-based biomaterials are at the forefront of biomedicine, presenting innovative solutions to longstanding challenges in tissue engineering and wound repair. These advanced materials demonstrate enhanced mechanical properties and improved biocompatibility while allowing for precise tuning of drug release profiles. Recent progress in the design, fabrication, and characterization of these biomaterials underscores their transformative potential in biomedicine. Researchers are employing strategic methodologies to investigate and characterize the structure and functionality of nanocellulose in tissue engineering and wound repair. In tissue engineering, nanocellulose-based scaffolds offer transformative opportunities to replicate the complexities of native tissues, facilitating the study of drug effects on the metabolism, vascularization, and cellular behavior in engineered liver, adipose, and tumor models. Concurrently, nanocellulose has gained recognition as an advanced wound dressing material, leveraging its ability to deliver therapeutic agents via precise topical, transdermal, and systemic pathways while simultaneously promoting cellular proliferation and tissue regeneration. The inherent transparency of nanocellulose provides a unique advantage, enabling real-time monitoring of wound healing progress. Despite these advancements, significant challenges remain in the large-scale production, reproducibility, and commercial viability of nanocellulose-based biomaterials. This review not only underscores these hurdles but also outlines strategic directions for future research, including the need for bioengineering of nanocellulose-based wound dressings with scalable production and the incorporation of novel functionalities for clinical translation. By addressing these key challenges, nanocellulose has the potential to redefine biomedical material design and offer transformative solutions for unmet clinical needs in tissue engineering and beyond.