Evidence map›Paper›PMID 40011255›Full record

ArticleJournal of molecular histology2025

Effects of simulated microgravity on dental pulp stem cell stemness.

Huailong Hou, Zhengjun Qiu, Jingyi Che, Yanping Li, Jingxuan Sun, Weiwei Zhang, Jinjie Ma, Shuang Zhang, Mengdi Li, Yumei Niu and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Huailong Hou *Department of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Zhengjun Qiu *Department of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Jingyi CheDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Yanping LiDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Jingxuan SunDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Weiwei ZhangDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Jinjie MaDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Shuang ZhangDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Mengdi LiDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China.
Yumei NiuDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China. niuym@hrbmu.edu.cn.
Lina HeDepartment of Endodontics, The First Affiliated Hospital of Harbin Medical University, 143 Yiman Street, St Nangang Dist., Harbin, 150001, China. helina426@163.com.

Funding

the Joint Guiding project of Heilongjiang Natural Science Foundation LH2022H043the Key Research and Development Program of Heilongjiang Province 2022ZX06C05the Young Medical Talent Training Funding Project of the First Affiliated Hospital of Harbin Medical University 2024YQ18
6 · The paper itself

Abstract

Dental pulp stem cells (DPSCs), a subset of tooth-derived mesenchymal stem cells (MSCs), demonstrate significant promise in clinical stem cell therapy. However, prolonged in vitro expansion commonly results in compromised stemness, limiting therapeutic efficacy. Thus, maintaining the stemness of DPSCs during expansion and culture is a key challenge for regenerative medicine. In the current study, the impact of simulated microgravity (SMG) on DPSC stemness was investigated using the three-dimensional clinostat Cellspace-3D. After SMG treatment for 3 days, DPSCs demonstrated markedly enhanced replicative activity, proliferation efficiency, self-renewal capacity, and effective inhibition of the senescence process. Under specific differentiation induction conditions, DPSCs in the SMG group exhibited superior osteogenic, adipogenic, chondrogenic, and neural differentiation potentials. Additionally, DPSCs exhibited higher expression levels of the MSC surface markers Stro-1 and CD146 and stemness maintenance-related genes Oct4, Nanog, and Sox2 in the SMG group compared to those from the normal gravity (NG) group. To elucidate the potential molecular mechanisms by which SMG influences the stemness of DPSCs, transcriptome sequencing of total RNA was performed, and identified that differentially expressed genes (DEGs) are closely associated with the MAPK signaling pathway. Further verification experiments demonstrated that the MAPK/ERK signaling pathway was activated in the SMG group. In conclusion, SMG effectively maintains the stemness of DPSCs cultivated in vitro, and its mechanism of action may be associated with the activation of the MAPK/ERK signaling pathway.

Indexed as

Dental PulpMesenchymal Stem CellsStem CellsWeightlessness SimulationCell DifferentiationCell ProliferationCells, CulturedHumansMAP Kinase Signaling SystemOsteogenesisWeightlessnessDental pulp stem cellsMAPK/ERK signaling pathwaySimulated microgravityStemness

Identifiers

PMID40011255

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.