Evidence map›Paper›PMID 40016501›Full record

ArticleThe AAPS journal2025

In Vitro-In Silico Models to Elucidate Mechanisms of Bile Acid Disposition and Cellular Aerobics in Human Hepatocytes.

Kristof De Vos, Raf Mols, Sagnik Chatterjee, Miao-Chan Huang, Patrick Augustijns, Justina Clarinda Wolters, Pieter Annaert

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Article in The AAPS journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kristof De VosDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, Herestraat 49, KU Leuven, 3000, Leuven, Belgium.ORCID 0000-0003-3280-2158
Raf MolsDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, Herestraat 49, KU Leuven, 3000, Leuven, Belgium.
Sagnik ChatterjeeDMPK Department, AstraZeneca, Västra Götaland County, Gothenburg, Sweden.
Miao-Chan HuangDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, Herestraat 49, KU Leuven, 3000, Leuven, Belgium.
Patrick AugustijnsDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, Herestraat 49, KU Leuven, 3000, Leuven, Belgium.
Justina Clarinda WoltersSection Systems Medicine of Metabolism and Signaling, Department of Pediatrics, University of Groningen, University Medical Center Groningen, Groningen, 9713 AV, The Netherlands.
Pieter AnnaertDrug Delivery and Disposition, Department of Pharmaceutical and Pharmacological Sciences, Herestraat 49, KU Leuven, 3000, Leuven, Belgium. pieter.annaert@kuleuven.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding the kinetics of hepatic processes, such as bile acid (BA) handling and cellular aerobic metabolism, is crucial for advancing our knowledge of liver toxicity, particularly drug-induced cholestasis (DiCho). This article aimed to construct interpretable models with parameter estimations serving as reference values when investigating these cell metrics. Longitudinal datasets on BA disposition and oxygen consumption rates were collected using sandwich-cultured human hepatocytes. Chenodeoxycholic acid (CDCA), lithocholic acid (LCA), as well as their amidated and sulfate-conjugated metabolites were quantified with liquid chromatography-mass spectrometry. The bile salt export pump (BSEP) abundance was monitored with targeted proteomics and modelled for activity assessment. Oxygen consumption was measured using Seahorse XFp analyser. Ordinary differential equation-based models were solved in R. The basolateral uptake and efflux clearance of glycine-conjugated CDCA (GCDCA) were estimated at 1.22 µL/min/10

Indexed as

Bile Acids and SaltsHepatocytesModels, BiologicalATP Binding Cassette Transporter, Subfamily B, Member 11Cells, CulturedChenodeoxycholic AcidComputer SimulationHumansOxygen ConsumptionATP Binding Cassette Transporter, Subfamily B, Member 11Bile Acids and SaltsChenodeoxycholic AcidBile acid dispositionBile salt export pumpMechanistic modelling and simulationMitochondrial respirationSandwich-cultured human hepatocytes

Identifiers

PMID40016501

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.