ArticleBiotechnology for biofuels and bioproducts2025
Design and characterization of allantoin-inducible expression systems in budding yeast.
Article in Biotechnology for biofuels and bioproducts, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Transcriptomics-guided identification of engineering targets for improving salt tolerance in Saccharomyces cerevisiae.Microbial cell factories · 2026Article
- Transposon engineering ofEngineering in life sciences · 2026Article
- Enhanced vitamin A production by engineering transporters, ATP and precursor supply.Biodesign research · 2025Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundSaccharomyces cerevisiae has been extensively employed as a host for the production of various biochemicals and recombinant proteins. The expression systems employed in S. cerevisiae typically rely on constitutive or galactose-regulated promoters, and the limited repertoire of gene expression regulations imposes constraints on the productivity of microbial cell factories based on budding yeast.
resultsIn this study, we designed and characterized a series of allantoin-inducible expression systems based on the endogenous allantoin catabolic system (DAL-related genes) in S. cerevisiae. We first characterized the expression profile of a set of DAL promoters induced by allantoin, and further combined with the galactose-inducible (GAL) system to create a highly responsive genetic switch that efficiently amplifies the output signals. The resulting allantoin-GAL system could give a ON/OFF ratio of 68.6, with 6.8-fold higher signal output over that of direct P
conclusionsIn summary, we characterized several allantoin-inducible DAL promoters from budding yeast and further developed a layered allantoin-GAL system that utilizes the DAL2 promoter to regulate the galactose regulon in budding yeast. The resulting allantoin-GAL system could give an impressive ON/OFF ratio that surpassed the traditional P
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.