Evidence map›Paper›PMID 40024368›Full record

ArticleBiomedical journal2025

A panel of miRNAs in serum extracellular vesicles serves as novel diagnostic biomarkers for MASLD.

Moran Hu, Hai Huang, Meng Jia, Min Xu, Malin Chen, Junxiang Wu, Shouyong Gu, Hongwei Liang, Hongwen Zhou, Yingyun Gong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
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  10. Urinary extracellular vesicle RNAs as novelClinical kidney journal · 2025
    Article
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  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Moran HuDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
Hai HuangDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China; Department of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Jiangsu, China.
Meng JiaState Key Laboratory of Pharmaceutical Biotechnology, Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing Medical University, Nanjing University, Jiangsu, China.
Min XuInstitute of Geriatric Medicine, Jiangsu Province Geriatric Hospital, Nanjing, Jiangsu, China.
Malin ChenThe First Clinical College of Nanjing Medical University, Jiangsu, China.
Junxiang WuDepartment of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Jiangsu, China.
Shouyong GuInstitute of Geriatric Medicine, Jiangsu Province Geriatric Hospital, Nanjing, Jiangsu, China.
Hongwei LiangDepartment of Emergency, Nanjing Drum Tower Hospital, School of Life Science and Technology, China Pharmaceutical University, Jiangsu, China. Electronic address: hwliang@cpu.edu.cn.
Hongwen ZhouDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China. Electronic address: drhongwenzhou@njmu.edu.cn.
Yingyun GongDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China. Electronic address: gongyingyun@njmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The rising global prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) poses a growing challenge to healthcare systems, stimulating substantial research efforts to develop reliable diagnostic methodologies. Emerging evidence highlights extracellular vesicles (EVs) as promising non-invasive biomarkers due to their roles in metabolic regulation and disease progression. This study investigated the diagnostic potential of serum EV-derived microRNAs (miRNAs) for MASLD detection and staging. We developed a novel diagnostic approach combining wheat germ agglutinin (WGA)-coupled magnetic beads for EV capture with RT-qPCR analysis, creating a streamlined two-step protocol that eliminates conventional purification requirements. MiR-574-3p, miR-542-3p, and miR-200a-3p in serum EVs were significantly elevated in patients with MASLD, indicating their potential as non-invasive biomarkers. Here, our established platform offers a clinically feasible solution for EVs isolation and quantitative miRNA analysis, presenting significant advantages in diagnostic efficiency and practical implementation.

Indexed as

Extracellular VesiclesFatty LiverMicroRNAsBiomarkersFemaleHumansMaleMiddle AgedBiomarkersMicroRNAsExtracellular vesiclesMASLDmiRNAWGA-coupled magnetic beads

Identifiers

PMID40024368
PMCPMC12704274

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.