Evidence map›Paper›PMID 40029010›Full record

ArticleBioconjugate chemistry2025

Scalable One-Pot Production of Geranylgeranylated Proteins in Engineered Prokaryotes.

Md Shahadat Hossain, Md Mahbubul Alam, Zhiwei Huang, Faeze Mousazadeh, Ronit Sarangi, Ebbing de Jong, Kavindu C Kolamunna, Albert L Adhya, James L Hougland, Atanu Acharya and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioconjugate chemistry, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Md Shahadat HossainDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.
Md Mahbubul AlamDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.
Zhiwei HuangDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.
Faeze MousazadehDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.
Ronit SarangiDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.
Ebbing de JongUpstate Medical University, Proteomics and Mass Spectrometry, Weiskotten Hall 4307 WHA, 766 Irving Avenue, Syracuse, New York 13210, United States.
Kavindu C KolamunnaDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.
Albert L AdhyaDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.
James L HouglandDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.ORCID 0000-0003-0444-1017
Atanu AcharyaDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.ORCID 0000-0002-6960-7789
Davoud MozhdehiDepartment of Chemistry, Syracuse University, 111 College Place, Syracuse, New York 13244, United States.ORCID 0000-0002-3440-8878

Funding

Genetically Encoded Lipidation to Manipulate Structure, Assembly, and Phase Behavior of ProteinsR35GM142899 · NIGMS · SYRACUSE UNIVERSITY · PI MOZHDEHI , DAVOUD · 2021 to 2025
$1.8M
Determining the scope of prenylatable protein sequencesR01GM132606 · NIGMS · UNIVERSITY OF GEORGIA · PI KANNAN, NATARAJAN, SCHMIDT, WALTER K · 2019 to 2022
$1.6M
Investigation of Long-Range Charge Transfer and Excited State Processes in Biochemical SystemsR35GM150874 · NIGMS · SYRACUSE UNIVERSITY · PI Atanu Acharya · 2023 to 2026
$1.5M
NIGMS NIH HHS R01 GM132606NIGMS NIH HHS R35 GM142899NIGMS NIH HHS R35 GM150874
6 · The paper itself

Abstract

Geranylgeranylation is a critical post-translational modification essential for various cellular functions. However, current methods for synthesizing geranylgeranylated proteins are complex and costly, which hinders access to these proteins for both biophysical and biomaterials applications. Here, we present a method for the one-pot production of geranylgeranylated proteins in

Indexed as

Escherichia coliProtein EngineeringProtein PrenylationPolyisoprenyl Phosphatesgeranylgeranyl pyrophosphatePolyisoprenyl Phosphates

Identifiers

PMID40029010
PMCPMC11926785

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.