Evidence map›Paper›PMID 40035725›Full record

ArticleInvestigative ophthalmology & visual science2025

Dicer Loss in Müller Glia Leads to a Defined Sequence of Pathological Events Beginning With Cone Dysfunction.

Daniel Larbi, Alexander M Rief, Seoyoung Kang, Shaoheng Chen, Khulan Batsuuri, Sabine Fuhrmann, Suresh Viswanathan, Stefanie G Wohl

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Daniel LarbiDepartment of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States.
Alexander M RiefDepartment of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States.
Seoyoung KangDepartment of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States.
Shaoheng ChenDepartment of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States.
Khulan BatsuuriDepartment of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States.
Sabine FuhrmannOphthalmology and Visual Sciences Department, Vanderbilt Eye Institute, Vanderbilt University Medical Center, Nashville, Tennessee, United States.
Suresh ViswanathanIndiana University School of Optometry, Bloomington, Indiana, United States.
Stefanie G WohlDepartment of Biological and Vision Sciences, The State University of New York College of Optometry, New York, New York, United States.

Funding

Regulation of Eye MorphogenesisR01EY024373 · NEI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI SABINE FUHRMANN · 2015 to 2026
$3.4M
Role of Wnt/Frizzled signaling during eye developmentR01EY014954 · NEI · UNIVERSITY OF UTAH · PI FUHRMANN, SABINE · 2003 to 2013
$3.4M
The Role of Retinal Progenitor microRNAs for Late-stage Progenitor Cell State and Muller Glia ReprogrammingR01EY032532 · NEI · STATE COLLEGE OF OPTOMETRY · PI Stefanie G Wohl · 2022 to 2026
$2.0M
Promoting RPE repair through modulation of Hippo signalingR21EY032724 · NEI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI FUHRMANN, SABINE · 2021 to 2022
$462k
NEI NIH HHS R01 EY014954NEI NIH HHS R01 EY024373NEI NIH HHS R01 EY032532NEI NIH HHS R21 EY032724
6 · The paper itself

Abstract

Purpose: The loss of Dicer in Müller glia (MG) results in severe photoreceptor degeneration, as it occurs in retinitis pigmentosa or age-related macular degeneration; however, the sequence of events leading to this severe degenerative state is unknown. The aim of this study was to conduct a chronological functional and structural characterization of the pathological events in MG-specific Dicer-conditional knockout (cKO) mice in vivo and histologically. Methods: To delete Dicer and mature microRNAs (miRNAs) in MG, two conditional Dicer1 knockout mouse strains (Rlbp-CreER:tdTomato:Dicer-cKOMG and Glast-CreER:tdTomato:Dicer-cKOMG) were created. Optical coherence tomography (OCT), electroretinograms (ERGs), and histological analyses were conducted to investigate structural and functional changes up to 6 months after Dicer deletion. Results: Dicer/miRNA loss in MG leads to (1) impairments of the area spanning from the external limiting membrane (ELM) to the retinal pigment epithelium (RPE), (2) cone photoreceptor dysfunction, and (3) retinal remodeling and functional loss of the inner retina at 1, 3, and 6 months after Dicer loss, respectively, in both of the knockout mouse strains. Furthermore, in the Rlbp-CreER:tdTomato:Dicer-cKOMG strain, rod photoreceptor impairment was found 4 months after Dicer depletion (4) accompanied by alteration of RPE integrity (5). Conclusions: MG Dicer loss in the adult mouse retina impacts cone function prior to any measurable changes in rod function, suggesting a pivotal role for MG Dicer and miRNAs in supporting cone health. A partially impaired RPE, however, seems to accelerate rod degeneration and overall degenerative events.

Indexed as

DEAD-box RNA HelicasesEpendymoglial CellsRetinal Cone Photoreceptor CellsRetinal DegenerationRibonuclease IIIAnimalsDisease Models, AnimalElectroretinographyMiceMice, KnockoutMicroRNAsRetinal Pigment EpitheliumTomography, Optical CoherenceDEAD-box RNA HelicasesDicer1 protein, mouseMicroRNAsRibonuclease III

Identifiers

PMID40035725
PMCPMC11892533

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.