Evidence map›Paper›PMID 40035996›Full record

ArticleCurrent medical science2025

Safranal Ameliorates Renal Damage, Inflammation, and Podocyte Injury in Membranous Nephropathy via SIRT/NF-κB Signalling.

Yan Bao, Ya-Mei Ge, Zheng Wang, Hong-Yun Wang, Qiong Wang, Jun Yuan

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Article in Current medical science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yan Bao *Department of Nephrology, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Ya-Mei Ge *The First Clinical College, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Zheng WangBSc Biochemistry, University College London, London, WCIE 6BT, UK.
Hong-Yun WangThe First Clinical College, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Qiong WangThe First Clinical College, Hubei University of Chinese Medicine, Wuhan, 430065, China.
Jun YuanDepartment of Nephrology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. 1927@hbucm.edu.cn.

Funding

Innovation Program of Wuhan-Basic Research in 2022 No.2022020801020506National Natural Science Foundation of China 82074364)National Natural Science Foundation of China (No.82474412Natural Science Foundation of Hubei Province 2022CFC024
6 · The paper itself

Abstract

objectiveSafranal is a natural product from saffron (Crocus sativus L.) with anti-inflammatory and nephroprotective potential. This study aimed to explore the role of safranal in a cationic bovine serum albumin (C-BSA)-induced rat model of membranous glomerulonephritis (MGN).

methodsAfter model establishment, Sprague-Dawley rats were administered 100 or 200 mg/kg safranal by gavage. A biochemical analyser was used to measure the urine protein levels and serum levels of renal function parameters. Hematoxylin-eosin and immunofluorescence staining of kidney tissues were performed to examine histopathological changes and assess the expression of IgG, C3, and Sirt1. Western blotting was performed to measure the protein levels of podocin, nephrin, Sirt1, and factors involved in the NF-κB/p65 pathway. Inflammatory cytokine levels in renal homogenates were determined by ELISA.

resultsSafranal at 100 or 200 mg/kg reduced kidney weight (2.07 ± 0.15 g and 2.05 ± 0.15 g) and the kidney somatic index (0.83 ± 0.08% and 0.81 ± 0.08%) in MGN rats compared with those in the model group without drug administration (2.62 ± 0.17 g and 1.05 ± 0.1%). C-BSA increased the urine protein level to 117.68 ± 10.52 mg/day (compared with the sham group, 5.03 ± 0.45 mg/day), caused dysregulation of renal function indicators, and induced glomerular expansion and inflammatory cell infiltration in the rat kidney samples. All the biochemical and histological changes were improved by safranal administration. Safranal at two doses also increased the fluorescence intensities of IgG (0.1 ± 0.009 and 0.088 ± 0.008) and C3 (0.065 ± 0.006 and 0.048 ± 0.004) compared with those in the MGN group (0.15 ± 0.013 and 0.086 ± 0.008). Additionally, safranal reversed the downregulation of podocin, nephrin, and Wilms tumor protein-1 (WT1) levels and reversed the high inflammatory cytokine levels in MGN rats. Mechanistically, safranal activated Sirt1 signalling to interfere with NF-κB signalling in the kidney tissues of MGN rats.

conclusionsSafranal ameliorates renal damage, inflammation, and podocyte injury in MGN by upregulating SIRT1 and inhibiting NF-κB signalling.

Indexed as

CyclohexenesGlomerulonephritis, MembranousInflammationPodocytesSirtuin 1TerpenesAnimalsDisease Models, AnimalIntracellular Signaling Peptides and ProteinsKidneyMaleMembrane ProteinsNF-kappa BRatsRats, Sprague-DawleySerum Albumin, BovineCyclohexenesIntracellular Signaling Peptides and ProteinsMembrane ProteinsnephrinNF-kappa BNPHS2 proteinsafranalSerum Albumin, BovineSirt1 protein, ratSirtuin 1TerpenesInflammationMembranous nephropathyNuclear factor kappa BSafranalSirtuin type-1

Identifiers

PMID40035996
PMCPMC12052800

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.