Evidence mapPaperPMID 40038378Full record

ArticleScientific reports2025

USP37-stabilized SALL4 promotes the keloid formation by PI3K/AKT pathway.

Shuo Fang, Zishuo Wang, Jianguo Xu, Miao Xu, Jiesong Zhou, Yuntong Zhang, Chunyu Xue

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shuo FangDepartment of Plastic Surgery, The First Affiliated Hospital of Navy Medical University, Shanghai City, 200000, P.R. China.
Zishuo WangSchool of Exercise and Health, Shanghai University of Sport, Shanghai City, 200000, P.R. China.
Jianguo XuDepartment of Plastic Surgery, The First Affiliated Hospital of Navy Medical University, Shanghai City, 200000, P.R. China.
Miao XuDepartment of Plastic Surgery, PLA Naval medical center, Shanghai City, 200000, P.R. China.
Jiesong ZhouDepartment of Plastic Surgery, The First Affiliated Hospital of Navy Medical University, Shanghai City, 200000, P.R. China.
Yuntong ZhangDepartment of Orthopedics and Trauma, The First Affiliated Hospital of Navy Medical University, Shanghai City, 200000, P.R. China. yuntongzhang2023@163.com.
Chunyu XueDepartment of Plastic Surgery, The First Affiliated Hospital of Navy Medical University, Shanghai City, 200000, P.R. China. Xcyfun@yeah.net.

Funding

National Natural Science Foundation of China 81701923
6 · The paper itself

Abstract

Spalt-like transcription factor 4 (SALL4) plays a vital role in the progression of many human diseases. However, the role and mechanism of SALL4 regulates in keloid formation remain unclear. The mRNA levels of SALL4 and ubiquitin-specific peptidase 37 (USP37) in keloid tissues and keloid fibroblasts were determined by quantitative real-time PCR. Western blot was performed to measure the protein levels of SALL4, USP4/10/37, collagen-related markers, and PI3K/AKT-related markers. The growth, invasion and migration of keloid fibroblasts were determined using CCK8 assay, EdU assay, flow cytometry, transwell assay and wound healing assay. Cell glycolysis was assessed by detecting glucose consumption and lactate production. The interaction between USP37 and SALL4 was confirmed by co-immunoprecipitation assay. SALL4 had increased expression in keloid tissues and keloid fibroblasts. Silencing of SALL4 inhibited the growth, invasion, migration, extracellular matrix (ECM) accumulate and glycolysis of keloid fibroblast, while its overexpression had the opposite effects. In terms of mechanism, USP37 stabilized SALL4 expression through deubiquitinating. Functional experiments suggested that SALL4 overexpression reversed the inhibitory effect of USP37 knockdown on keloid fibroblast functions. Moreover, USP37/SALL4 axis could increase the activity of PI3K/AKT pathway, and PI3K pathway inhibitor LY294002 abolished SALL4-mediated the promoting on keloid fibroblast functions. USP37-activated SALL4 might enhance keloid fibroblast growth, invasion, migration, ECM accumulation and glycolysis via activating PI3K/AKT pathway.

Indexed as

KeloidPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionTranscription FactorsUbiquitin-Specific ProteasesAdultCell MovementCell ProliferationFemaleFibroblastsGlycolysisHumansMalePhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSALL4 protein, humanTranscription FactorsUbiquitin-Specific ProteasesKeloid fibroblastsPI3K/AKTSALL4USP37

Identifiers

PMID40038378
PMCPMC11880302

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.