Evidence map›Paper›PMID 40040812›Full record

ArticleiScience2025

Exploring NLRP3-related phenotypic fingerprints in human macrophages using Cell Painting assay.

Matthew Herring, Eva Särndahl, Oleksandr Kotlyar, Nikolai Scherbak, Magnus Engwall, Roger Karlsson, Mikael Ejdebäck, Alexander Persson, Andi Alijagic

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Matthew HerringSchool of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Eva SärndahlSchool of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Oleksandr KotlyarMan-Technology-Environment Research Center (MTM), Örebro University, Örebro, Sweden.
Nikolai ScherbakMan-Technology-Environment Research Center (MTM), Örebro University, Örebro, Sweden.
Magnus EngwallMan-Technology-Environment Research Center (MTM), Örebro University, Örebro, Sweden.
Roger KarlssonDepartment of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden.
Mikael EjdebäckSchool of Bioscience, Systems Biology Research Centre, University of Skövde, Skövde, Sweden.
Alexander PerssonSchool of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.
Andi AlijagicSchool of Medical Sciences, Faculty of Medicine and Health, Örebro University, Örebro, Sweden.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Existing research has proven difficult to understand the interplay between upstream signaling events during NLRP3 inflammasome activation. Additionally, events downstream of inflammasome complex formation such as cytokine release and pyroptosis can exhibit variation, further complicating matters. Cell Painting has emerged as a prominent tool for unbiased evaluation of the effect of perturbations on cell morphological phenotypes. Using this technique, phenotypic fingerprints can be generated that reveal connections between phenotypes and possible modes of action. To the best of our knowledge, this was the first study that utilized Cell Painting on human THP-1 macrophages to generate phenotypic fingerprints in response to different endogenous and exogenous NLRP3 inflammasome triggers and to identify phenotypic features specific to NLRP3 inflammasome complex formation. Our results demonstrated that not only can Cell Painting generate morphological fingerprints that are NLRP3 trigger-specific but it can also identify cellular fingerprints associated with NLRP3 inflammasome activation.

Indexed as

Biological sciences research methodologiesCell biologyImmunology

Identifiers

PMID40040812
PMCPMC11876907

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.