Evidence map›Paper›PMID 40042274›Full record

ArticleApplied and environmental microbiology2025

Computer-directed rational engineering of dioxygenase TcsAB for triclosan biodegradation under cold conditions.

Yiran Yin, Xinjie Yu, Zongxin Tao, Christopher E French, Zhenmei Lu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yiran YinMOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, China.ORCID 0000-0002-7087-8789
Xinjie YuMOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, China.
Zongxin TaoMOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, China.
Christopher E FrenchZhejiang University-University of Edinburgh Joint Research Centre for Engineering Biology, International Campus, Zhejiang University, Haining, China.
Zhenmei LuMOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, China.ORCID 0000-0003-4768-9522

Funding

National Key Research and Development Program of China 2021YFA0909500National Natural Science Foundation of China 32370117Zhejiang Provincial Natural Science Foundation of China LZ24C010003Zhejiang Provincial Xinmiao Talents Program (Zhejiang Xinmiao Talents Program) 2024R401191
6 · The paper itself

Abstract

The dioxygenase TcsAB is a specific dioxygenase involved in the initial biodegradation of the broad-spectrum antibacterial agent triclosan (TCS). However, it exhibits significantly reduced activity under cold conditions. In this study, a computer-directed approach combining loop engineering and N-terminal truncation was utilized to decrease the thermostability of TcsAB, thereby enhancing its catalytic activity in cold environments. The iterative mutant TcsAB (TcsA

Indexed as

Bacterial ProteinsDioxygenasesPseudomonasTriclosanBiodegradation, EnvironmentalCold TemperatureMolecular Dynamics SimulationProtein EngineeringBacterial ProteinsDioxygenasesTriclosanbiodegradationcold catalysisdioxygenaserational engineeringtriclosan

Identifiers

PMID40042274
PMCPMC12016537

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.