ArticleParasites & vectors2025
Risk assessment of an Aedes flavivirus and its effect on pathogenic flavivirus replication in mosquitoes.
Article in Parasites & vectors, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Host and geography shape microbial communities in Kenyan mosquitoes: insights from metatranscriptomics.mSystems · 2026Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
backgroundMosquitoes are efficient vectors of medically significant flaviviruses and serve as hosts for insect-specific flaviviruses (ISFs). Aedes flavivirus (AEFV) is a classical ISF. Given the increasing discovery of ISFs, it is urgent to evaluate the potential risk of ISFs to human health as well as their impact on the transmission of pathogenic flaviviruses.
methodsWe isolated a strain of AEFV from wild Aedes albopictus populations in Hainan Province, China, using iodixanol density-gradient ultracentrifugation. The infection of the AEFV Hainan strain in Aedes, Culex, and four mammalian cell lines was investigated using fluorescence in situ hybridization (FISH) assays, and relative and absolute quantitative polymerase chain reaction (qPCR). Whether AEFV alters the vector competence of Ae. albopictus for pathogenic arboviruses and the underlying immune mechanisms were explored.
resultsThe AEFV Hainan strain showed close genetic similarity to strains from Yunnan province of China, Thailand, and Peru. This strain was capable of infecting Ae. albopictus and Ae. aegypti but not Culex quinquefasciatus. Cell entry was the critical barrier for AEFV infection in Cx. quinquefasciatus cells. The infection risk of the AEFV Hainan strain in four mammalian cells (BHK-21, Vero, 293 T, and HeLa) was quite low due to the failure of cell entry or extremely limited replication. Prior infection of AEFV was detrimental to the replication of Zika virus and dengue virus serotype 2 in Ae. albopictus through activation of the Janus kinase/signal transducer and activator of transcription, Toll, or RNA interference pathway.
conclusionsOur work excludes the risk of the AEFV Hainan strain to human health and highlights its potential as an immune inducer to sabotage Aedes mosquito ability for viral transmission.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.