Evidence map›Paper›PMID 40048003›Full record

ArticlePlanta2025

Identification and characterization of a key gene controlling purple leaf coloration in non-heading Chinese cabbage (Brassica rapa).

Jia Si, Xiaoqing Zhou, Xinyu Chen, Huilin Ming, Hanqiang Liu, Maixia Hui

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Fine-Mapping ofBiology · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jia SiVegetables Engineering and Technology Research Center of Shaanxi Province, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. sijia@nwafu.edu.cn.
Xiaoqing ZhouVegetables Engineering and Technology Research Center of Shaanxi Province, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China. 2392744124@qq.com.
Xinyu ChenVegetables Engineering and Technology Research Center of Shaanxi Province, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Huilin MingVegetables Engineering and Technology Research Center of Shaanxi Province, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Hanqiang LiuVegetables Engineering and Technology Research Center of Shaanxi Province, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Maixia HuiDepartment of Vegetable Science, College of Horticulture, Northwest A&F University, No. 3 Taicheng Road, Yangling Demonstration Zone, Xianyang, Shaanxi, China. maixiahui@163.com.

Funding

Xi'an science and technology research project 23NYGG0026
6 · The paper itself

Abstract

MAIN

conclusionChalcone isomerase (BraCHI, BraA03g059660.3C) is the candidate gene controlling purple leaf coloration in non-heading Chinese cabbage. A 10-bp deletion in its promoter enhances gene expression in purple plants, likely by disrupting MYB transcription factor binding, leading to anthocyanin accumulation. Leaf color is a critical trait influencing the commercial and nutritional value of leafy vegetables, with purple-leafed varieties prized for their high anthocyanin content. In this study, we investigated the genetic basis of purple leaf coloration in non-heading Chinese cabbage (Brassica rapa). Using a recombinant inbred line (RIL) population derived from a cross between purple-leafed S45P and green-leafed S45G lines, bulked segregant analysis sequencing (BSA-seq) and fine mapping were performed. The analysis identified BraP2, a locus on chromosome A03 associated with purple leaf coloration. Within the 65.31 kb candidate region, BraA03g059660.3C, encoding chalcone isomerase (CHI), was identified as the strongest candidate gene. Quantitative real-time PCR (qRT-PCR) revealed significantly higher expression of BraA03g059660.3C in purple-leafed S45P plants compared to green-leafed S45G plants. Further sequence analysis uncovered a 10-bp deletion in the promoter region of BraA03g059660.3C in S45P plants. This deletion likely disrupts a MYB transcription factor binding site, enhancing gene expression and promoting anthocyanin accumulation. Our findings demonstrate that BraA03g059660.3C plays a pivotal role in controlling purple leaf coloration in non-heading Chinese cabbage. This discovery advances the understanding of anthocyanin biosynthesis regulation and provides valuable genetic resources for breeding Brassica crops with improved esthetic and nutritional qualities.

Indexed as

Brassica rapaIntramolecular LyasesPigmentationPlant LeavesPlant ProteinsAnthocyaninsChromosome MappingGene Expression Regulation, PlantGenes, PlantPromoter Regions, GeneticAnthocyaninschalcone isomeraseIntramolecular LyasesPlant ProteinsAnthocyaninBSA-seqChalcone isomeraseGene mappingNon-heading Chinese cabbage

Identifiers

PMID40048003

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.