Evidence map›Paper›PMID 40064138›Full record

ArticleBiomaterials2025

Investigation of the protein corona and biodistribution profile of polymeric nanoparticles for intra-amniotic delivery.

Anna Y Lynn, Kwangsoo Shin, David A Eaton, Micky Rose, Xianzhi Zhang, Madalina Ene, Julian Grundler, Emily Deschenes, Rachel Rivero, Laura G Bracaglia and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Systemic in utero gene editing as a treatment for cystic fibrosis.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Anna Y LynnDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA.
Kwangsoo ShinDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA.
David A EatonDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA.
Micky RoseDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA.
Xianzhi ZhangDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA.
Madalina EneDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA.
Julian GrundlerDepartment of Chemistry, Yale University, New Haven, CT, 06510, USA.
Emily DeschenesDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA.
Rachel RiveroDepartment of Surgery, Yale School of Medicine, New Haven, CT, 06510, USA.
Laura G BracagliaDepartment of Chemical and Biological Engineering, Villanova University, Villanova, PA, 19085, USA.
Peter M GlazerDepartment of Therapeutic Radiology, Yale School of Medicine, New Haven, CT, 06510, USA; Department of Genetics, Yale School of Medicine, New Haven, CT, 06510, USA.
David H StitelmanDepartment of Surgery, Yale School of Medicine, New Haven, CT, 06510, USA.
W Mark SaltzmanDepartment of Biomedical Engineering, Yale University, New Haven, CT, 06510, USA; Department of Chemical & Environmental Engineering, Yale University, New Haven, CT, 06510, USA; Department of Cellular & Molecular Physiology, Yale University, New Haven, CT, 06510, USA; Department of Dermatology, Yale School of Medicine, New Haven, CT, 06510, USA. Electronic address: mark.saltzman@yale.edu.

Funding

Poly(amine-co-ester)s for Targeted Delivery In Vivo of Gene Editing Agents to Bone Marrow and LungUG3HL147352 · NHLBI · YALE UNIVERSITY · PI GLAZER, PETER M, SALTZMAN, W. MARK · 2018 to 2020
$2.4M
Poly(amine-co-ester)s for targeted delivery of gene editing agents to treat cystic fibrosis in animal models: SCGE Disease Models Studies SupplementUH3HL147352 · NHLBI · YALE UNIVERSITY · PI GLAZER, PETER M, SALTZMAN, W. MARK · 2021 to 2022
$2.4M
Engineering of Polymeric Particles for Fetal TherapyR01EB032791 · NIBIB · YALE UNIVERSITY · PI W. Mark Saltzman, David Stitelman · 2023 to 2026
$1.7M
NHLBI NIH HHS UG3 HL147352NHLBI NIH HHS UH3 HL147352NIBIB NIH HHS R01 EB032791
6 · The paper itself

Abstract

When exposed to the biological environment, nanoparticles (NPs) form a protein corona that influences delivery profile. We present a study of protein corona formation and NP biodistribution in amniotic fluid (AF) for poly(lactic-co-glycolic acid) (PLGA) and poly(lactic-acid) (PLA) NPs, with and without polyethylene glycol (PEG), as well as poly(amine-co-ester)-PEG (PACE-PEG) NPs. The presence of surface PEG and polyvinyl alcohol (PVA) were characterized to investigate surfactant role in determining protein corona formation. The surface density of PEG groups demonstrated an inverse correlation with the total amount of protein surface adsorption. All PEGylated NPs exhibited a dense brush conformation and demonstrated higher levels of stability in AF than non-PEGylated NPs. The protein corona composition varied by core polymer, while the amount of protein adsorption varied by PEGylation status. In A549 cells, in vitro cellular association of each NP type correlated with the amount of albumin that was found in the protein corona. In vivo, only PEGylated NPs were able successfully distribute to fetal organs, likely due to the enhanced stability imparted by PEG. PLGA-PEG and PACE-PEG NPs had both high levels of albumin in the protein corona and high biodistribution to the fetal lung, consistent with the association with lung cells in vitro. PLA-PEG NPs distributed exclusively to the fetal bowel, which we propose is associated with known gastrointestinal targeting keratin proteins. By furthering our understanding of polymeric NP behavior in AF, this novel study provides a basis for optimization of intra-amniotic NP delivery systems targeting congenital pulmonary and gastrointestinal diseases.

Indexed as

Amniotic FluidNanoparticlesPolymersProtein CoronaA549 CellsAdsorptionAnimalsFemaleHumansPolyestersPolyethylene GlycolsPolylactic Acid-Polyglycolic Acid CopolymerPregnancyTissue DistributionPolyestersPolyethylene GlycolsPolylactic Acid-Polyglycolic Acid CopolymerPolymersProtein CoronaFetal therapyHuman amniotic fluidIntra-amniotic deliveryPolymeric nanoparticlesProtein corona

Identifiers

PMID40064138
PMCPMC11972154

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.