Article in Advanced healthcare materials, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
16 authors.
Mrunal PatilAix Marseille Université, CNRS, CINaM UMR 7325, Campus de Luminy, Case 913, Cedex 09, Marseille, 13288, France.ORCID 0000-0002-5722-6567
Tatiana MunteanuAix Marseille Université, CNRS, CINaM UMR 7325, Campus de Luminy, Case 913, Cedex 09, Marseille, 13288, France.ORCID 0000-0002-3682-6838
Gaël BrasseurLaboratoire de Chimie Bactérienne, Institut de Microbiologie de la Méditerranée, CNRS, Aix-Marseille Université, Marseille, 13402, France.ORCID 0000-0002-5490-270X
Carolina FerreiraGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Lisbon, 1349-008, Portugal.ORCID 0000-0003-3070-7421
Sofia Santos CostaGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Lisbon, 1349-008, Portugal.ORCID 0000-0002-4096-2410
Isabel CoutoGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Lisbon, 1349-008, Portugal.ORCID 0000-0003-3536-2733
Mohd AtharPhysics Department, University of Cagliari, SP 8, km 0.700, Monserrato, 09042, Cagliari, Italy.ORCID 0000-0001-6337-1026
Elisa AsunisPhysics Department, University of Cagliari, SP 8, km 0.700, Monserrato, 09042, Cagliari, Italy.
Attilio Vittorio VargiuPhysics Department, University of Cagliari, SP 8, km 0.700, Monserrato, 09042, Cagliari, Italy.ORCID 0000-0003-4013-8867
Miguel ViveirosGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Lisbon, 1349-008, Portugal.ORCID 0000-0001-9676-6251
Carole DiGiorgioLaboratoire de Mutagénèse Environnementale, Aix-Marseille Université, CNRS, IRD, Avignon Université, IMBE UMR 7263, Marseille, 13385, France.ORCID 0000-0002-4962-3191
Frédéric BrunelAix Marseille Université, CNRS, CINaM UMR 7325, Campus de Luminy, Case 913, Cedex 09, Marseille, 13288, France.
Jean-Manuel RaimundoAix Marseille Université, CNRS, CINaM UMR 7325, Campus de Luminy, Case 913, Cedex 09, Marseille, 13288, France.ORCID 0000-0003-4090-0479
Michel CamploAix Marseille Université, CNRS, CINaM UMR 7325, Campus de Luminy, Case 913, Cedex 09, Marseille, 13288, France.ORCID 0000-0002-4446-0661
Olivier SiriAix Marseille Université, CNRS, CINaM UMR 7325, Campus de Luminy, Case 913, Cedex 09, Marseille, 13288, France.ORCID 0000-0001-9747-3813
Optimization of efflux avoidance and inhibition for antibiotic developmentR01AI136799 · NIAID · UNIVERSITY OF OKLAHOMA · PI GNANAKARAN, SANDRASEGARAM, RUGGERONE, PAOLO · 2018 to 2022
$5.7M
A*Midex Foundation Interdisciplinarity GrantCNRS-Innovation "Phenux"DREBI 2022.07931.PTDCDREBI CEECINST/00042/2021/CP1773/CT0009DREBI UI/BD/154472/2022Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa UI/BD/154472/2022Fundação para a Ciência e a TecnologiaGHTM UID/04413/2020LA-REAL LA/P/0117/2020LA-REAL PTDC/CAL-EST/30713/2017NIAID NIH HHS R01 AI136799
6 · The paper itself
Abstract
Efflux-mediated antibiotic resistance poses a significant global threat, affecting diverse bacterial species. Clinicians recognize the danger of efflux mechanisms during antibiotic treatment, yet precise diagnostic tools remain unavailable. The antibiogram currently infers abnormal efflux pump activity in clinical isolates, which is subsequently confirmed through transcriptomic or genomic analysis. This study harnesses the colorimetric, fluorescence, and solubility properties of phenazinium derivatives to develop a rapid protocol for detecting bacterial efflux. Among several synthesized phenazinium compounds, the compound demonstrating differential MIC in Staphylococcus efflux mutants and exhibiting appropriate physicochemical properties is selected. A diagnostic protocol is developed using the selected compound to assess efflux levels, categorized as no, weak, or strong, through colorimetry and spectroscopy techniques. Testing on Gram-positive efflux mutants and clinical Staphylococcus isolates further validates the approach. In-silico docking analysis confirms the interaction between the chosen compound and the NorA efflux pump in S. aureus. Flow cytometry is employed to re-evaluate the detection assays. The developed molecule and protocol represent the first known method to evaluate efflux levels in any Gram-positive species through a streamlined and user-friendly process. This pioneering test significantly advances the epidemiological analysis of efflux mechanisms and enables more informed therapeutic decision-making, leading to more effective treatment.
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.
Unlocking the Gates: A Novel Diagnostic Molecule for Quantifying Efflux Levels in Gram-Positive Bacteria. · full record | Socratic