Evidence map›Paper›PMID 40067621›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Experimental Neurogenesis in the Embryos of the Gecko Paroedura picta.

Sara Jiménez, Rodrigo Senovilla-Ganzo, Tatiana Gallego-Flores, Erise Pérez-Pascual, Aitor Ordeñana-Manso, Raquel Rayo-Morales, Maurizio De Pittà, Fernando García-Moreno

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sara JiménezAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain. sara.jimenez@achucarro.org.ORCID 0000-0001-9043-9049
Rodrigo Senovilla-GanzoAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain.
Tatiana Gallego-FloresAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain.
Erise Pérez-PascualAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain.
Aitor Ordeñana-MansoAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain.
Raquel Rayo-MoralesAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain.
Maurizio De PittàDepartment of Neuroscience, Faculty of Medicine and Odontology, UPV/EHU, Leioa, Spain.
Fernando García-MorenoAchucarro Basque Center for Neuroscience, Scientific Park of the University of the Basque Country (UPV/EHU), Leioa, Spain. fernando.garcia-moreno@achucarro.org.ORCID 0000-0001-9048-4237

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This research introduces innovative tools for studying embryonic neurogenesis in the gecko Paroedura picta. Traditional research methods have been adapted for the gecko's unique biology, including variations of birthdating techniques and the implementation of EdU for tracking neuron generation. We also employ hybridization chain reaction (HCR) to detect specific mRNAs without the need for species-specific antibodies, providing a powerful and flexible tool for studying gene expression patterns in the gecko embryonic brain. However, HCR can also be combined with immunohistochemistry to visualize both RNA and protein distribution. Our work proves the importance of this innovative toolset to understand the evolutionary aspects of nervous system development and the gecko as a valuable model for understanding the ancestral stem amniote.

Indexed as

Embryo, NonmammalianLizardsNeurogenesisAnimalsBrainGene Expression Regulation, DevelopmentalImmunohistochemistryNeuronsRNA, MessengerRNA, MessengerBrain evolutionEdU birthdatingEmbryonic developmentGeckoHybridization chain reaction (HCR)Neurogenesis

Identifiers

PMID40067621

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.