Evidence map›Paper›PMID 40071078›Full record

ArticleSovremennye tekhnologii v meditsine2025

Simultaneous Application of Methylene Blue and Chlorin e6 Photosensitizers: Investigation on a Cell Culture.

A V Ryabova, I D Romanishkin, I V Markova, D V Pominova

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sovremennye tekhnologii v meditsine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

A V RyabovaSenior Researcher, Laser Biospectroscopy Laboratory, Light-Induced Surface Phenomena Department, Natural Sciences Center; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia; Associate Professor, Department 87 "Laser Micro-, Nano-, and Biotechnologies, Engineering Physics Institute for Biomedicine"; National Research Nuclear University MEPhI, 31 Kashirskoye Highway, Moscow, 115409, Russia.
I D RomanishkinJunior Researcher, Laser Biospectroscopy Laboratory, Light-Induced Surface Phenomena Department, Natural Sciences Center; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia.
I V MarkovaPhD Student; National Research Nuclear University MEPhI, 31 Kashirskoye Highway, Moscow, 115409, Russia; Engineer, Laser Biospectroscopy Laboratory, Light-Induced Surface Phenomena Department, Natural Sciences Center; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia.
D V PominovaPhD, Senior Researcher, Laser Biospectroscopy Laboratory, Light-Induced Surface Phenomena Department, Natural Sciences Center; Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov St., Moscow, 119991, Russia; Associate Professor, Department 87 "Laser Micro-, Nano-, and Biotechnologies, Engineering Physics Institute for Biomedicine"; National Research Nuclear University MEPhI, 31 Kashirskoye Highway, Moscow, 115409, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The application of photosensitizers for inhibition of oxidative phosphorylation in order to temporally decrease oxygen uptake by tumor cells in the course of photodynamic therapy (PDT) evokes growing interest. Material and Methods: A photodynamic activity of MB and its combined use with chlorin e6 has been studied on the HeLa cell culture, their effect on cell metabolism in their co-accumulation and subsequent irradiation has also been assessed. Results: MB generates reactive oxygen species in the cells in contrast to chlorin e6, which produces singlet oxygen. Besides, MB is converted to a colorless leucoform at low concentrations in the process of de-oxygenation. Incubation of cells with MB concurrently with chlorin e6 results in its greater fluorescence as compared to the incubation with MB only. MB concentration in the range of 1-10 mg/kg and the laser radiation dose of 60 J/cm Conclusion: The co-accumulation of MB with chlorin e6 prevents MB reduction to a colorless leucoform, decreasing the oxygen uptake by the cells and making it possible to use simultaneously type I and II photodynamic reactions.

Indexed as

Methylene BluePhotochemotherapyPhotosensitizing AgentsPorphyrinsChlorophyllidesHeLa CellsHumansReactive Oxygen SpeciesChlorophyllidesMethylene BluePhotosensitizing AgentsphytochlorinPorphyrinsReactive Oxygen Speciesaerobic glycolysischlorin e6hypoxiamethylene blueoxidative phosphorylationphotodynamic therapy

Identifiers

PMID40071078
PMCPMC11892575

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.