Evidence map›Paper›PMID 40074292›Full record

ArticlePlant biotechnology journal2025

RPT: An integrated root phenotyping toolbox for segmenting and quantifying root system architecture.

Jiawei Shi, Shangyuan Xie, Weikun Li, Xin Wang, Jianglin Wang, Yunyu Chen, Yongyue Chang, Qiaojun Lou, Wanneng Yang

Abstract read
In one paragraph

Article in Plant biotechnology journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jiawei Shi *National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Shangyuan Xie *National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.ORCID https://orcid.org/0009-0007-4618-4035
Weikun Li *National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Xin WangNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Jianglin WangNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Yunyu ChenNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Yongyue ChangNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Qiaojun LouShanghai Agrobiological Gene Center, Shanghai, China.
Wanneng YangNational Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.ORCID https://orcid.org/0000-0003-1095-1355

Funding

Fundamental Research Funds for the Central Universities 2662024ZKPY003Key Agricultural Technology Research Project in Hubei Province HBNYHXGG2023-9National Key Research and Development Program of China 2022YFD2002304National Natural Science Foundation of China U21A20205
6 · The paper itself

Abstract

The dissection of genetic architecture for rice root system is largely dependent on phenotyping techniques, and high-throughput root phenotyping poses a great challenge. In this study, we established a cost-effective root phenotyping platform capable of analysing 1680 root samples within 2 h. To efficiently process a large number of root images, we developed the root phenotyping toolbox (RPT) with an enhanced SegFormer algorithm and used it for root segmentation and root phenotypic traits. Based on this root phenotyping platform and RPT, we screened 18 candidate (quantitative trait loci) QTL regions from 219 rice recombinant inbred lines under drought stress and validated the drought-resistant functions of gene OsIAA8 identified from these QTL regions. This study confirmed that RPT exhibited a great application potential for processing images with various sources and for mining stress-resistance genes of rice cultivars. Our developed root phenotyping platform and RPT software significantly improved high-throughput root phenotyping efficiency, allowing for large-scale root trait analysis, which will promote the genetic architecture improvement of drought-resistant cultivars and crop breeding research in the future.

Indexed as

OryzaPlant RootsDroughtsPhenotypePlant BreedingQuantitative Trait LociSoftwaredeep learninghigh‐throughput phenotyping platformrice drought resistanceroot phenotypingroot system architecture

Identifiers

PMID40074292
PMCPMC12120892

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.