Evidence mapPaperPMID 40079446Full record

ArticleCNS neuroscience & therapeutics2025

Proteomic Analyses of Clots Identify Stroke Etiologies in Patients Undergoing Endovascular Therapy.

Tae Jung Kim, Jin Woo Jung, Young-Ju Kim, Byung-Woo Yoon, Dohyun Han, Sang-Bae Ko

Abstract read
In one paragraph

Article in CNS neuroscience & therapeutics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Tae Jung KimDepartment of Neurology, Seoul National University College of Medicine, Seoul, Korea.ORCID 0000-0003-3616-5627
Jin Woo JungTransdisplinary Department of Medicine & Advanced Technology, Seoul National University Hospital, Seoul, Korea.
Young-Ju KimDepartment of Neurology, Seoul National University College of Medicine, Seoul, Korea.
Byung-Woo YoonDepartment of Neurology, Uijeongbu Eulji Medical Center, Uijeongbu, Korea.
Dohyun HanTransdisplinary Department of Medicine & Advanced Technology, Seoul National University Hospital, Seoul, Korea.
Sang-Bae KoDepartment of Neurology, Seoul National University College of Medicine, Seoul, Korea.

Funding

Seoul National University Hospital 0320210030
6 · The paper itself

Abstract

aimsThis study aimed to investigate the correlation between clot composition and stroke mechanisms in patients undergoing endovascular therapy (EVT), using proteomic analysis.

methodsThis study included 35 patients with ischemic stroke (cardioembolism [CE], n = 17; large artery atherosclerosis [LAA], n = 6; cancer-related [CR], n = 4; and undetermined (UD) cause, n = 8) who underwent EVT. Retrieved clots were proteomically analyzed to identify differentially expressed proteins associated with the three stroke mechanisms and to develop the machine learning model.

resultsIn the discover stage, 3838 proteins were identified using clot samples from 27 patients with CE, LAA, and CR mechanisms. Through functional enrichment and network analysis, 149 proteins were identified as potential candidates for verification studies. After verification experiments, 34 proteins were selected as the final candidates to predict stroke mechanisms. Furthermore, the machine learning-based model identified three proteins associated with each mechanism (Pleckstrin in CE; CD59 glycoprotein in LAA; and Immunoglobulin Heavy Constant Gamma 1 in CR) in the UD group.

conclusionsThis study identified specific protein markers of clots that could differentiate stroke mechanisms in patients undergoing EVT. Therefore, our results could offer valuable insights into elucidating the mechanisms of ischemic stroke, which could provide information on more effective secondary prevention strategies.

Indexed as

Endovascular ProceduresIschemic StrokeProteomicsStrokeThrombosisAgedAged, 80 and overBiomarkersFemaleHumansMachine LearningMaleMiddle AgedBiomarkersbiomarkerclotproteomic analysisstroke mechanisms

Identifiers

PMID40079446
PMCPMC11904956

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