Evidence map›Paper›PMID 40083175›Full record

ArticleG3 (Bethesda, Md.)2025

A genome assembly and annotation for the Australian alpine skink Bassiana duperreyi using long-read technologies.

Benjamin J Hanrahan, Kirat Alreja, Andre L M Reis, J King Chang, Duminda S B Dissanayake, Richard J Edwards, Terry Bertozzi, Jillian M Hammond, Denis O'Meally, Ira W Deveson and 3 more

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
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  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Benjamin J HanrahanSchool of Biotechnology and Biomolecular Science, Faculty of Science, University of New South Wales, Sydney, Sydney, NSW 2052, Australia.ORCID 0000-0001-9567-0071
Kirat AlrejaJohn Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia.ORCID 0009-0007-8937-9844
Andre L M ReisGenomics and Inherited Disease Program, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia.
J King ChangSchool of Biotechnology and Biomolecular Science, Faculty of Science, University of New South Wales, Sydney, Sydney, NSW 2052, Australia.ORCID 0009-0007-8748-4368
Duminda S B DissanayakeInstitute for Applied Ecology, University of Canberra, Bruce, ACT 2617, Australia.ORCID 0000-0002-7307-4639
Richard J EdwardsSchool of Biotechnology and Biomolecular Science, Faculty of Science, University of New South Wales, Sydney, Sydney, NSW 2052, Australia.ORCID 0000-0002-3645-5539
Terry BertozziSouth Australian Museum, Adelaide, SA 5000, Australia.ORCID 0000-0001-6665-3395
Jillian M HammondGenomics and Inherited Disease Program, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia.
Denis O'MeallyArthur Riggs Diabetes & Metabolism Research Institute, City of Hope, Duarte, CA 91024, USA.ORCID 0000-0001-7749-9506
Ira W DevesonGenomics and Inherited Disease Program, Garvan Institute of Medical Research, Sydney, NSW 2010, Australia.ORCID 0000-0003-3861-0472
Arthur GeorgesInstitute for Applied Ecology, University of Canberra, Bruce, ACT 2617, Australia.ORCID 0000-0003-2428-0361
Paul WatersSchool of Biotechnology and Biomolecular Science, Faculty of Science, University of New South Wales, Sydney, Sydney, NSW 2052, Australia.ORCID 0000-0002-4689-8747
Hardip R PatelJohn Curtin School of Medical Research, The Australian National University, Canberra, ACT 2601, Australia.ORCID 0000-0003-3169-049X

Funding

AusARG initiativeAustralian Research Council DP220101429Bioplatforms Australia
6 · The paper itself

Abstract

The eastern 3-lined skink (Bassiana duperreyi) inhabits the Australian high country in the southeast of the continent including Tasmania. It is a distinctive oviparous species because it undergoes sex reversal (from XX genotypic females to phenotypic males) at low incubation temperatures. We present a chromosome-scale genome assembly of a B. duperreyi XY male individual, constructed using PacBio HiFi and Oxford Nanopore Technologies long reads scaffolded using Illumina HiC data. The genome assembly length is 1.57 Gb with a scaffold N50 of 222 Mb, N90 of 26 Mb, 200 gaps, and 43.10% GC content. Most (95%) of the assembly is scaffolded into 6 macrochromosomes, 8 microchromosomes, and the X chromosome, corresponding to the karyotype. Fragmented Y chromosome scaffolds (n = 11 ≥ 1 Mb) were identified using Y-specific contigs generated by genome subtraction. We identified 2 novel alpha-satellite repeats of 187 and 199 bp in the putative centromeres that did not form higher-order repeats. The genome assembly exceeds the standard recommended by the Earth BioGenome Project: 0.02% false expansions, 99.63% k-mer completeness, 94.66% complete single-copy Benchmarking Universal Single-Copy Orthologs genes, and an average 98.42% of transcriptome data mappable to the genome assembly. The mitochondrial genome (17,506 bp) and the model rDNA repeat unit (15,154 bp) were assembled. The B. duperreyi genome assembly has high completeness for a skink and will provide a resource for research focused on sex determination and thermolabile sex reversal, as an oviparous foundation species for studies of the evolution of viviparity and for other comparative genomics studies of the Scincidae.

Indexed as

GenomeGenomicsLizardsMolecular Sequence AnnotationAnimalsAustraliaFemaleMalegenome assemblyNanoporePacBiosex reversalskink

Identifiers

PMID40083175
PMCPMC12134992

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.