Evidence map›Paper›PMID 40093039›Full record

ArticlebioRxiv : the preprint server for biology2025

Inwardly rectifying potassium channels regulate membrane potential polarization and direction sensing during neutrophil chemotaxis.

Tianqi Wang, Daniel H Kim, Chang Ding, Dingxun Wang, Weiwei Zhang, Martin Silic, Xi Cheng, Kunming Shao, TingHsuan Ku, Conwy Zheng and 6 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Tianqi WangDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Daniel H KimDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Chang DingDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Dingxun WangDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA.
Weiwei ZhangDepartment of Botany and Plant Pathology, Purdue University, West Lafayette, IN47907, USA.
Martin SilicDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA.
Xi ChengDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Kunming ShaoDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
TingHsuan KuDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Conwy ZhengDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Junkai XieDavidson School of Chemical Engineering, Purdue University, West Lafayette, IN47907, USA.
Chongli YuanDavidson School of Chemical Engineering, Purdue University, West Lafayette, IN47907, USA.
Alexander ChubykinDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Christopher J StaigerDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
Guangjun ZhangDepartment of Comparative Pathobiology, Purdue University, West Lafayette, IN 47907, USA.
Qing DengDepartment of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.ORCID 0000-0002-9254-9951

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI ANDREW D MESECAR · 1985 to 2026
$43.4M
Role of microRNAs in neutrophil migrationR35GM119787 · NIGMS · PURDUE UNIVERSITY · PI Qing Deng · 2016 to 2026
$4.9M
Roles of ion-channel mediated bioelectricity in developmental patterningR35GM124913 · NIGMS · PURDUE UNIVERSITY · PI GuangJun Zhang · 2017 to 2026
$4.2M
NCI NIH HHS P30 CA023168NIGMS NIH HHS R35 GM119787NIGMS NIH HHS R35 GM124913
6 · The paper itself

Abstract

Potassium channels regulate membrane potential and diverse physiological processes, including cell migration. However, the specific function of the inwardly rectifying potassium channels in immune cell chemotaxis is unknown. Here, we identified that the inwardly rectifying potassium channel Kir7.1 (KCNJ13) maintains the resting membrane potential and is required for directional sensing during neutrophil chemotaxis. Pharmacological or genetic inhibition of Kir7.1 in neutrophils impaired direction sensing toward various chemoattractants without affecting cell polarization in multiple neutrophil models. Using genetically encoded voltage indicators, we observed oscillating depolarization of the membrane potential in protrusions in zebrafish neutrophils, and Kir7.1 is required for polarized depolarization towards the chemokine source. Focal depolarization with optogenetic tools biases pseudopod selection and induces

Indexed as

bioelectricitydirection sensingKir7.1zebrafish

Identifiers

PMID40093039
PMCPMC11908270

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.