Evidence map›Paper›PMID 40093124›Full record

ArticlebioRxiv : the preprint server for biology2025

Dissecting the molecular landscape of Parkinson's disease and Parkinson's disease dementia using highly efficient snRNA-seq (HIF-snRNA-seq).

Sung-Ung Kang, Jinhee Park, Shinwon Ha, Dongsan Kim, Olga Pletnikova, Javier Redding-Ochoa, Juan C Troncoso, Quan Peng, Beth O Van Emburgh, Jaldhir Trivedi and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Sung-Ung KangNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205 USA.ORCID 0000-0002-8393-2588
Jinhee ParkNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205 USA.
Shinwon HaNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205 USA.
Dongsan KimNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205 USA.
Olga PletnikovaDepartment of Pathology (Neuropathology), Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.ORCID 0000-0001-6516-4944
Javier Redding-OchoaDepartment of Pathology (Neuropathology), Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.ORCID 0000-0002-0677-917X
Juan C TroncosoDepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205 USA.ORCID 0000-0001-9553-6673
Quan PengValted Seq, 704 Quince Orchard Rd, Suite 320, Gaithersburg, MD 20878 USA.
Beth O Van EmburghValted Seq, 704 Quince Orchard Rd, Suite 320, Gaithersburg, MD 20878 USA.
Jaldhir TrivediValted Seq, 704 Quince Orchard Rd, Suite 320, Gaithersburg, MD 20878 USA.
Saurav BrahmachariValted Seq, 704 Quince Orchard Rd, Suite 320, Gaithersburg, MD 20878 USA.ORCID 0009-0004-3056-6213
Bardia NezamiValted Seq, 704 Quince Orchard Rd, Suite 320, Gaithersburg, MD 20878 USA.
Valina L DawsonNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205 USA.
Ted M DawsonNeuroregeneration and Stem Cell Programs, Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, MD, 21205 USA.ORCID 0000-0002-6459-0893

Funding

Research Education ComponentP30AG066507 · NIA · JOHNS HOPKINS UNIVERSITY · PI Corinne Pettigrew · 2020 to 2026
$29.3M
Region-Specific Vulnerability of the Lewy Body Dementia's BrainR01NS123456 · NINDS · JOHNS HOPKINS UNIVERSITY · PI KAM, TAE-IN, KANG, SUNG UNG · 2021 to 2025
$3.6M
NIA NIH HHS P30 AG066507NINDS NIH HHS R01 NS123456
6 · The paper itself

Abstract

This study presents a transcriptomic analysis of the cingulate cortex (CING) in Parkinson's disease (PD) and Parkinson's disease dementia (PDD) using a High-efficiency single-nucleus RNA sequencing (HiF-snRNA-seq) protocol optimized for post-mortem brain samples. RNA quality prediction, poly-A tailing, and dCas9-targeted depletion enabled analysis of 77 high-quality samples from 240 cases, yielding over 2 million nuclei classified into seven major cell types. Disease conditions revealed altered astrocyte and microglia proportions, implicating their roles in neuroinflammation. Differential expression analysis identified unique and shared genes across PD and PDD, linked to synaptic remodeling, stress responses, and inflammation. Stage-specific analysis uncovered tau-dependent early-stage genes and inflammation-associated late-stage genes. This study highlights the CING's central role in PD and PDD pathophysiology, offering insights into disease mechanisms and identifying candidate genes and pathways for therapeutic and biomarker development.

Identifiers

PMID40093124
PMCPMC11908213

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.