Gestational high-sucrose diet mediated vascular hyper-contractility in mesenteric arteries from offspring.

Prenatal high sucrose diet (HS) generates profound effects on vascular diseases in offspring later in life. This study aimed to determine whether and how prenatal HS affect vasoreactivity in resistance arteries from adult offspring. Pregnant Sprague-Dawley rats were fed with normal drinking water or 20% high-sucrose solution during the whole gestational period. Mesenteric arteries (MAs) from adult offspring were obtained and tested for vascular functions with DMT. The whole-transcriptome sequencing (RNA-seq) of MAs was performed to reveal the different genes and possible pathway. Real-time PCR and western blot were performed to access mRNA and protein expression. The thicker smooth muscle layer and mitochondrial swelling were observed in MAs in HS offspring. Prenatal HS mediated higher vasoconstriction/vascular sensitivity induced by phenylephrine (PE) and 5-Hydroxytryptamine (5-HT). RNA-Seq analysis revealed that the genes crystallin alpha B (CYRAB) and heat shock protein family E member 1 (HSPE1) were upregulated, while the gene adenomatous polyposis coli downregulated 1 (APCDD1) was downregulated in HS group, confirmed at mRNA and protein expression levels. Wingless-related integration site (Wnt)/Ca