ReviewNature biotechnology2025
Toward single-molecule protein sequencing using nanopores.
Review in Nature biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Advancing the applications of liquid biopsies in oncology.Nature reviews. Genetics · 2026Review
- Article
- Exopeptidase-assisted nanopore peptide sequence identification.Nature communications · 2026Article
- Tools and tactics for studying alternative splicing.Nature reviews. Genetics · 2026Review
- Article
- Large-Diameter DNA-Scaffolded Nanopores Enabled by Loosely Packed Peptides for Single-Molecule Sensing.Angewandte Chemie (International ed. in English) · 2026Article
- Epx4 Nanopore With Multiple Constrictions for Single-Molecule Identification.Small methods · 2026Article
- Single-Molecule Peptide Discrimination via Flow-Through SERS and Machine Learning.ACS photonics · 2026Article
- Generalizable Direct Protein Sequencing With InstaNexus.Molecular & cellular proteomics : MCP · 2026Article
- Label-Free Optical Differentiation of Single Diffusing Amino Acids at Picomolar Concentrations.Research square · 2026Article
- Scalable Production of DNA-Probe-Functionalized Heteromeric MspA Nanopores for Biosensing.ACS applied materials & interfaces · 2026Article
- Nanopipette-DNA-Nanopore Hybrid System for Small Molecule Detection.ChemPlusChem · 2026Article
- Background-Free Nanopore Decoding of microRNA Expression Patterns Using Circular Diagnostic DNA.Analytical chemistry · 2026Article
- A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Single-Molecule Discrimination of Multisialylated Ganglioside Oligosaccharides Using an Engineered Nanopore.Research (Washington, D.C.) · 2026Article
- Purification and Oligomerization of YaxAB Nanopores.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Construction and characterization of a nanopore derived from the transmembrane domain of a trimeric autotransporter adhesin.Frontiers in bioengineering and biotechnology · 2026Article
- Fabrication and Characterization of Glass Nanopores.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Absence of Hofmeister Selectivity in Hydrophobic Ion-Exchanger Nanopores.Analytical chemistry · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Over the past three decades, biological nanopore sequencing has grown from a research curiosity to a mature technology to sequence nucleic acids at the single-molecule level. Now, recent achievements suggest that nanopores might be able to sequence proteins soon. In this Perspective, we analyze the different approaches that have been proposed to measure proteins and peptides using nanopores. We predict that, more likely than not, nanopores will be capable of identifying full-length proteins at the single-molecule level and with single-amino acid resolution, paving the way to single-molecule protein sequencing. This would allow several applications in proteomics that are at present challenging, including measuring the heterogeneity of post-translational modifications, quantifying low-abundance proteins and characterizing protein splicing.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.