Evidence mapPaperPMID 40100580Full record

ArticleDiscover oncology2025

Development and validation of M2 macrophage-related genes in a prognostic model of lung adenocarcinoma based on bulk RNA and ScRNA datasets.

Bolin Wang, Xiaofeng Zhou, Di Wu, Lu Gao, Zhihua Wan, Ruifeng Wu

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In one paragraph

Article in Discover oncology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Bolin WangGraduate School of Chengde Medical College, Chengde, Hebei, China.
Xiaofeng ZhouDepartment of Chest Surgery, Baoding First Central Hospital, Baoding, Hebei, China.
Di WuDepartment of Chest Surgery, Baoding First Central Hospital, Baoding, Hebei, China.
Lu GaoDepartment of Chest Surgery, Baoding First Central Hospital, Baoding, Hebei, China.
Zhihua WanDepartment of Chest Surgery, Baoding First Central Hospital, Baoding, Hebei, China.
Ruifeng WuDepartment of Chest Surgery, Baoding First Central Hospital, Baoding, Hebei, China. 1477716025@qq.com.

Funding

Key Laboratory for Pulmonary Nodule Diagnosis and Treatment in Baoding 2463P023
6 · The paper itself

Abstract

objectiveThis study aimed to investigate the correlation between M2 macrophages activity with the prognosis of lung adenocarcinoma (LUAD). We sought to identify key genes associated with M2 macrophage activity and examine their relationship with clinicopathological features to elucidate the underlying mechanism.

methodsPublished datases were analyzed for differentially expressed genes. After quality control, batch effect removal, and annotation, the scRNA dataset identified M2 macrophage-associated differentially expressed genes in the LUAD group, which were cross-analyzed and referred to as M2 macrophage-linked genes. A risk model was generated using machine learing for these genes. Thereafter, two bulk RNA-seq datasets were used to evaluate the model. We computed risk scores for all samples and grouped them into low and high risk, aiding in the comparison of clinical characteristics, immune and stromal infiltration, and drug sensitivity. Finally, key genes were validated through immunohistochemistry in IPA samples.

resultsWe identified four key M2 macrophage-linked genes: TIMP1, CAV2, MIF, and SELENBP1. Survival durations in the high-riskscore cluster were lower across the TCGA-LUAD (P = 1.2 × 10

conclusionThe role of M2 macrophages in tumor progression could anticipate the prognosis of LUAD and develop novel immunotherapy strategies.

Indexed as

Lung adenocarcinomaM2 macrophagesRisk scoreSingle-cell sequencing

Identifiers

PMID40100580
PMCPMC11920479

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.