Evidence map›Paper›PMID 40100581›Full record

ArticleGenes & genomics2025

Molecular cloning, expression analysis, and functional characterization of Intelectin-1 from Chinese giant salamanders (Andrias davidianus).

Haolin Mo, Kexin Liu, Xiaoran An, Yongqing Chen, Jiajia Yu, Huixia Yu, Mingxing Yao, Weijia Song, Yang Li, Lixin Wang

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Article in Genes & genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Haolin MoCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Kexin LiuCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Xiaoran AnFisheries and Fisheries Administration of Department of Agriculture and Rural Affairs of Shaanxi Province, Xi'an, 710003, Shaanxi, China.
Yongqing ChenCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Jiajia YuCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Huixia YuCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Mingxing YaoCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Weijia SongCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Yang LiCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China.
Lixin WangCollege of Animal Science and Technology, Northwest A&F University, Xinong Road, Yangling, 712100, Shaanxi, China. fisherwanglx@nwsuaf.edu.cn.

Funding

National Natural Science Foundation of China 32202909Natural Science Foundation of Shaanxi Province 2022JQ-204
6 · The paper itself

Abstract

backgroundIntelectin (ITLN) plays a pivotal role in innate immunity, inflammatory responses, and tumor progression. However, its physiological roles in caudate amphibians, such as Andrias davidianus, remain elusive.

objectiveA proto-type of intelectin (AdITLN1) from A. davidianus was characterized, following which an AdITLN1 homology model was generated to analyze its expression profiles and functional characterizations.

methodsThe intelectin1 gene (AdITLN1) was cloned, and its evolutionary relationship with ITLN1 from other species was explored. Additionally, a homology model was generated using Molecular Operating Environment (MOE) software, and the active binding pocket of AdITLN1 was identified. Infections with Aeromonas hydrophila were conducted to analyze changes in ITLN1 transcript levels in liver tissue. Finally, recombinant A. davidianus ITLN1 protein (rAdITLN1) was synthesized using prokaryotic expression, and its bacterial agglutination activity and impact on macrophage phagocytosis were examined.

resultsMultiple sequence alignment and phylogenetic analysis indicated that AdITLN1 shared the closest evolutionary relationship with amphibians, with its structure being similar to that of human intelectin1 and Xenopus Embryonic Epidermal Lectin. Moreover, AdITLN1 mRNA was expressed in a wide range of tissues and was significantly up-regulated post-A. hydrophila infection. Meanwhile, the AdITLN1 protein was successfully expressed and purified in Escherichia coli (E. coli) BL21 (DE3). The recombinant AdITLN1 (rAdITLN1) displayed strong agglutination activity against different Gram-negative and Gram-positive bacteria. Lastly, The phagocytosis of rAdITLN1-treated E. coli by macrophages was significantly enhanced.

conclusionThe results of the present study demonstrated that AdITLN1 was a multifunctional immune protein with potent immunomodulatory activity. This study offers valuable insights into disease control in giant salamanders and the conservation of natural resources.

Indexed as

Amphibian ProteinsCaudataLectinsAeromonas hydrophilaAmino Acid SequenceAnimalsCloning, MolecularPhagocytosisPhylogenyAmphibian ProteinsLectinsAgglutinationChinese giant salamanderImmune responseIntelectin-1Phagocytosis

Identifiers

PMID40100581

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.