Evidence map›Paper›PMID 40106189›Full record

ArticleProbiotics and antimicrobial proteins2026

PotF Affects the Antibacterial Activity of Plantaricin BM-1 Against Escherichia coli K12 by Modulating Biofilm Formation and Cell Membrane Integrity.

Shichun Wang, Yawen Wang, Congyang Cheng, Hongxing Zhang, Junhua Jin, Xiaona Pang, Xiaodong Song, Yuanhong Xie

Abstract read
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In one paragraph

Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shichun Wang *Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China.
Yawen Wang *Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China.
Congyang ChengKey Laboratory of Dairy Quality Digital Intelligence Monitoring Technology, State Administration for Market Regulation, Inner Mongolia Mengniu Dairy (Group) Co., Ltd., Hohhot, China.
Hongxing ZhangLaboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China.
Junhua JinLaboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China.
Xiaona PangLaboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China.
Xiaodong SongKey Laboratory of Dairy Quality Digital Intelligence Monitoring Technology, State Administration for Market Regulation, Inner Mongolia Mengniu Dairy (Group) Co., Ltd., Hohhot, China. songxiaodong@mengniu.cn.
Yuanhong XieLaboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China. xieyuanh@163.com.

Funding

Classification Development of Beijing Municipal Universities-Construction of Urban Agriculture and Forestry Characteristic Teacher Team 11000024T000002961733
6 · The paper itself

Abstract

Plantaricin BM-1 exhibits antibacterial activity against Escherichia coli; however, the underlying mechanism remains unclear. This study aimed to investigate the function of PotF, a putrescine-binding protein, in regulating the antibacterial activity of plantaricin BM-1 against E. coli K12. The antibacterial activity of plantaricin BM-1 against E. coli K12 and JW0838 cells was assessed using growth curves. The differences in biofilm formation between the two E. coli strains were evaluated by crystal violet staining and confocal laser scanning microscopy. The effects of plantaricin BM-1 on E. coli morphology and cell membrane integrity were investigated by electron microscopy and lactate dehydrogenase release assays. Proteomics was used to screen for differentially expressed proteins (DEPs) that are potentially involved in regulating the antibacterial mechanism. The null mutation of potF enhanced the antibacterial effects of plantaricin BM-1 on E. coli, and caused a significant decrease (p < 0.05) in the biofilms of E. coli JW0838. The plantaricin disrupted the cell membrane of E. coli JW0838. Proteomic analysis revealed that potF mutation significantly affected several DEPs involved in biofilm formation. Plantaricin BM-1 exhibited significantly enhanced antibacterial activity against biofilm-associated gene mutants compared to wild-type E. coli K12. These findings enhance our understanding of the bacteriostasis of class IIa bacteriocins against Gram-negative microorganisms.

Indexed as

Anti-Bacterial AgentsBacterial ProteinsBacteriocinsBiofilmsCarrier ProteinsCell MembraneEscherichia coli K12Escherichia coli ProteinsAnti-Bacterial AgentsBacterial ProteinsBacteriocinsCarrier ProteinsEscherichia coli ProteinsBiofilm formationClass IIa bacteriocinsEscherichia coliPotFProteome

Identifiers

PMID40106189

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.